Effects of drought stress on physiological characteristics of rhizome of Drynaria roosii
Objective In order to analyze the physiological mechanism of the rhizome of Drynaria roosii in response to drought stress,the rhizome of D.roosii was used as experimental material to observe the changes of physiological characteristics under drought stress.Methods Using vermiculite as the substrate,the D.roosii with plant height of 10～20 cm,rhizome length of 3～10 cm and diameter of 1～3 cm was transplanted into a pot with a diameter of 18.5 cm,and cultivated in an artificial climate chamber.The cultivation temperature is 25℃,the relative humidity is 75％,the photoperiod is 12 h light/12 h darkness,and the light intensity is 80 pmol·m-2s-1.The plants were rejuvenated for7 days.D.roosii plants were treated with solutions containing different PEG-6000 concentra-tions(0％,5％,15％,25％,50％)for 7 days.And the morphological and physiological characteristics of the plants were observed.Results With the increase of drought treatment concentration,the water content of the rhizome decreased gradually,and the osmotic protective substances content,membrane permeability,active oxygen content and antioxidant enzyme activity increased gradually.Con-clusion Drought stress induces osmotic stress in the rhizome of D.roosii,which further leads to oxidative stress.The D.roosii rhizome regulates cell osmotic pressure by synthesizing osmotic protective substances,and scavenges reactive oxygen species by antioxidant en-zyme.
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