Mechanism research of experienced treatment"Sanqi-Longxuejie"for hyperlipidemia of professor Duan Fujin
Objective To investigate the mechanism of action of Sanqi-Longxuejie in the treatment of hyperlipidemia by net-work pharmacology and molecular docking technology combined with animal experiments.Methods The mechanism of action of Sanqi-Longxuejie in the treatment of hyperlipidemia was predicted by network pharmacology and preliminarily verified by mo-lecular docking.A mouse model of hyperlipidemia was constructed by high-fat feed feeding,lipid deposition in liver tissues of mice was observed by oil red staining,and the expression of key pathway-related proteins in the four levels of blood lipids and network pharmacology was detected by enzyme-linked immunosorption(ELISA)and Western Blot,so as to verify the mecha-nism of action of Panax notoginseng-dragon's blood exhaustion in the treatment of hyperlipidemia.Results A total of 17 active ingredients of Sanqi-Longxuejie,including quercetin,glycyrrhizin and naringenin,were screened by network pharmacology,corresponding to471 targets,2502 disease targets,and 158 drug and disease targets,including AKT1,VEGFR,EGFR,CASP3,PPARG and other core targets.GO enrichment analysis yielded 2134 biological processes such as steroid metabolism,kinase ac-tivity regulation,lipid metabolism regulation,87 cell components such as membrane raft,membrane microdomain,vesicle cavity,and 209 molecular functions such as nuclear receptor activity,steroid binding,and monocarboxylic acid binding.KEGG enrich-ment analysis yielded 163 pathways,including PI3K-AKT,HIF-1,and MAPK.The results of molecular docking showed that the five core components such as quercetin and the five core targets such as AKT1 had high binding energy.The results of animal experiments showed that Panax notoginseng blood depletion could effectively reduce lipid deposition in liver tissues of model mice,regulate blood lipid levels,inhibit the activity of lipid metabolism enzyme HMGR and the expression of PI3K,p-AKT and SREBP2 proteins in liver tissues.Conclusion Sanqi-Longxuejie can regulate the lipid metabolism function of hyperlipidemia mice by regulating the expression of proteins related to PI3K/AKT/SREBP2 signaling pathway,thereby improving the blood lipid level of mice.
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