Exploring the molecular mechanism of Triptolide in anti-renal cancer via data mining and in vitro experiments
Objective To explore the molecular mechanism of triptolide against renal carcinoma.Methods The potential target of triptolide in renal carcinoma was analyzed by network pharmacology and machine learning.The effect of triptolide on ACHN cell viability was detected by CCK-8 method,the effect of triptolide on ACHN cell migration inhibition was detected by scratch test,and the effect of triptolide on ACHN cell apoptosis was detected by flow cytometry.The expression difference of important target proteins was detected by Western blot.Results Network pharmacology predicted that triptolide inhibited the growth of renal carci-noma cells by STAT3,EGFR,AKT1,BCL2,mTOR and other core targets.The results of CCK-8 showed that the survival rate of ACHN cells decreased significantly with the increase of drug concentration(P＜0.001),and 10nM was the best inhibitory concentration.After triptolide treatment,the mobility of the experimental group decreased by 10.4％(P＜0.01)and the apop-tosis rate increased by 5.08％(P＜0.05).Western Blot showed that the expression levels of p-mTOR,P53 and LC3I/Ⅱ in-creased,while the expression levels of pSTAT3 and P62 decreased.Conclusion Triptolide can inhibit the proliferation and migra-tion of ACHN cells and induce autophagy apoptosis of renal carcinoma cells.
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