Basic Study on the Intervention of Qianliexiao Decoction in the Regulation of the Inflam-matory Axis of Caspase-1/IL-1 β/NF-κB p65 in NLRP3 Inflammasome in Chronic nonbacterial prostatitis Model mice
Objective To investigate the mechanism of Qianliexiao decoction regulating experimental autoimmune prostatitis through Caspase-1/IL-1β/NF-κB p65 pathway by intervening in NLRP3 inflammasomes.Methods The 120 male SPF-grade C57BL/6 mice were divided into blank group,model group,positive control group(MCC950),and high/medium/low dose group of Qianliexiao decoction according to the random number table method.Except for the blank group,the rest were prepared by composite factor molding method to prepare EAP mouse models,and the model was evaluated by dampness-heat syndrome scores,prostate index,HE staining pathology.Immunofluorescence detected the expression levels of NLRP3,Caspase-1 and IL-1β in prostate tissues of each group.Western Blot detected the expression levels of NLRP3,ASC,Caspase-1,GSDMD,NF-κB p65,and NF-κB p65 phosphorylated proteins in each group of mouse tissue.Results The dampness-heat syndrome scores and prostate index of the prostate of the model was higher than that in the blank group(P＜0.05).Compared with the blank group,a large number of inflammatory cell infiltration,glandular cavity structure disorder and fibrosis hyperplasia were seen in the glands and interstitium of the model group,and the degree of inflammatory infiltration of the prostate gland in the model group could be improved to different degrees,with MCC950 group and prostatic elimination and high-dose groups being the most significant.Immunofluorescence displayed that compared with the blank group,the expression of prostate NLRP3,Caspase-1 and IL-1 β in the model group was significantly enhanced(P＜0.01),and compared with the model group,the high/medium/low dose group of MCC950 group and the high/medium/low dose group of the model mouse could weaken the prostate NLRP3,Caspase-1 and IL-1β(P＜0.05)to varying degrees;compared with the prostatic neutralization/high dose group,the differ-ence was not statistically significant.Western Blot showed that compared with the blank group,the expression of prostate NLRP3,ASC,Caspase-1,GSDMD,NF-κB p65 and NF-κB p65 phosphorylated proteins in the model group was significantly in-creased(P＜0.01),and compared with the model group,the MCC950 group could downregulate the prostate NLRP3,ASC,Caspase-1,GSDMD,NF-κB p65,NF-κB to varying degrees in the model group The expression of p65 phosphorylated pro-tein(P＜0.01),and the dose group of Qianliexiao decoction was lowered to the medium/high dose group(P＜0.01).Conclu-sion Qianliexiao decoction can intervene in caspase-1/IL-1 mediated by NLRP3 inflammasome β/NF-κ B p65 inflammatory axis plays an intervention role in inhibiting inflammatory response of chronic nonbacterial prostatitis.
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