Effect of Compound Uncaria Jiangya Tablets on P2Y6 Receptor and Inflammatory Factor Levels in Spontaneous Hypertensive Rats
Objective To observe the effect of Compound Uncaria Jiangya Tablet on the expression of P2Y6 receptor and inflam-mation levels in SHR by measuring relevant indicators.Methods 60 SHRs were randomly divided into 5 groups,namely model control group,valsartan group,blocker group,uncaria hook group,and traditional Chinese and Western medicine group.The other 12 WKY rats were set as blank control group.After 8 weeks of continuous intervention according to the corresponding treat-ment methods,HMI and LVMI were measured,HE staining was used to observe left ventricular myocardial morphology,and im-munohistochemistry was used to detect the protein expression of IL-1β,IL-6 in the heart,RT-PCR determination the mRNA expression of IL-6,TNF-α,IL-1β,P2Y6,Western Blotting assaying the protein expression of P2Y6,IL-6,TNF-α,IL-1β.Results 1.Spontaneous hypertensive rats have significantly increased ventricular weight and left ventricular mass com-pared to normal rats,making them as a good research model for hypertensive LVH.2.Inflammatory factors IL-6,TNF-α,IL-1β increased in spontaneously hypertensive rats,indicating an increase in inflammatory activity in spontaneously hypertensive rats.3.Blocking P2Y6 receptors,the serum concentration of IL-6,TNF-α and IL-1β,as well as the mRNA and protein expression were significantly reduced too,indicating that P2Y6 receptor may be a key site of inflammatory response in spontane-ously hypertensive rats.4.After treatment with Compound Uncaria Hypotension Tablets,the serum concentration of IL-6,TNF-α,IL-1β decreased,as well as the mRNA and protein expression,suggesting that Compound Uncaria Jiangya Tablets can to some extent block the expression of P2Y6 receptors and exhibit synergistic effects with the Valsartan.Conclusion Compound Gouteng Jiangya tablets can extent block the expression of P2Y6 receptors,thereby reducing the level of chronic inflammation in SHR.
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