Study on Extraction of Scopoletin in Impatiens balsumina L.and Purification
Objective To select the best extraction process of scopoletin and the purification process of macroporous resin and study its adsorption mechanism.Methods The content of scopoletin was determined by HPLC and the influence of extraction tem-perature,ethanol concentration and time based on the orthogonal test design method.The types of macroporous resin were screened to optimize the enrichment and purification process and analyze the adsorption mechanism.Results The optimal extrac-tion process of ultrasound was 60 ℃,ethanol concentration of 80％ and extraction time of 30 min;HPD-100 macroporous resin has good purification ability for scopoletin,the adsorption dynamics meets the quasi-secondary kinetic model and the isothermal adsorption data meet the Freundich model.The best purification process is 60％ of 4 BV/h,of 4 BV/h,water eluting volume and 6 BV.The transfer rate was 73.46％ and the purity increased 7.64 times.Conclusion The preferred ultrasonic extraction process and the purification process of macroporous resin are stable and reliable.
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