To investigate the neural repair mechanism of Rougan Tongluo Decoction in cerebral is-chemia-reperfusion rats based on Notch pathway
Objective To study the effect of Rougan Tongluo Decoction on the expression of related proteins at different time points in the Notch signaling pathway in the hippocampus of rats with cerebral ischemia reperfusion,to explore the molecular regu-lation mechanism of Rougan Tongluo Decoction in the treatment of ischemic stroke.Methods 164 healthy male SD rats,Except for the blank Control group,other groups were Subjected to the preparation of focal ischemia-reperfusion(MCAO)using the thread embolism method(the Sham operation group did not form an infarction area).According to weight,they were randomly divided in-to blank control group,sham operation group,model group,Rougan Tongluo decoction group(Rougan group),except for the Rougan group,use Rougan Tongluo Decoction and the remaining three groups were all intragastrically administered with distilled water(1.4g/100g).Each group was divided into 5 subgroups according to the 5 time points 6h,1d,3d,7d,14d after 2h ische-mia and reperfusion,and 6 rats in each group.The neurological deficit was scored at 5 time points,and the brain was taken for TTC staining to assess the area of ischemic brain damage,and immunohistochemistry(SP method)was used to detect the protein expression of Notch1,Hes5,and Mash1 at different time points.Results Compared with the model group,the TTC stained cere-bral infarction area in the soft liver group was significantly lower than that in the model group(P＜0.05).Compared with the blank group and the sham operation group,the protein expressions of Notch1,Hes5,and Mash1 in the model group and the soft liver group increased from 3d to 14d after the operation(P＜0.05).Compared with the model:3d-14d after operation,the nerve defect score of the soft liver group was significantly decreased(P＜0.05),the protein expression of Notch1 and Hes5 in the liver softening group increased from 3d to 14d after operation compared with the model group(P＜0.05),and both protein ex-pressions decreased gradually after reaching the highest value on the 7th day after operation.The expression of Mash1 protein in the soft liver group was lower than that in the model group after 3d-14d(P＜0.05),the expression of Mash1 protein increased on the 3rd day,and decreased significantly on the 7th day,and reached the highest value on the 14th day.Conclusion Rougan Tongluo Decoction can improve the area of cerebral infarction and neurological dysfunction after ischemia by activating the Notch signaling pathway.Up-regulate the expression of Notch1 and Hes5 and down-regulate the expression of Mash1 in the early stage of cerebral ischemia injury,intervene in the cell cycle of neural stem cells,play a role in nerve repair and cell regeneration.
NETL
NSTL
万方数据
