HPLC-DAD波长切换法同时测定仙芎骨康颗粒中淫羊藿苷、朝藿定C、宝藿苷Ⅰ、阿魏酸的含量
Simultaneous Determination of Icariin,Epimedin C,Baohuoside Ⅰ and Ferulic Acid From Xianxionggukang Particles by HPLC-DAD
叶怡馨 1商佳璐 1汪轩 1章建华 2尹华1
作者信息
- 1. 浙江中医药大学药学院,浙江杭州 311402
- 2. 浙江中医药大学附属第一医院,浙江杭州 310006
- 折叠
摘要
目的 建立HPLC-DAD波长切换法同时测定仙芎骨康颗粒中淫羊藿苷、朝藿定C、宝藿苷Ⅰ、阿魏酸的含量.方法 采用多波长切换法,色谱柱:Zorbax Eclipse SB-C18,以乙腈-1％冰醋酸水为流动相梯度洗脱,流速1.0 mL·min-1,柱温30℃.结果 仙芎骨康颗粒中淫羊藿苷等4成分的峰面积与进样量均呈良好的线性关系(r＞0.999);平均加样回收率为98.9％～102.4％(RSD＜3.0％,n=6).3批样品中淫羊藿苷、朝藿定C、宝藿苷Ⅰ、阿魏酸含量限度分别为3.852、3.919、0.355 3、0.935 8 mg·g-1.结论 该方法简便快捷,结果准确可靠,方法重复性好,可用于仙芎骨康颗粒的质量控制,为制定质量标准草案提供依据.
Abstract
Objective To establish the simultaneous determination of icariin,epimedin C,baohuoside Ⅰ and ferulic acid from Xianxionggukang particles by HPLC-DAD.Methods A multi wavelength switching method was used,the chromatographic col-umn was Zorbax Eclipse SB-C18,with acetonitrile-1％glacial acetic acid water as the mobile phase gradient elution,flow rate 1.0 mL·min-1,column temperature 30 ℃.Results The peak areas of patchouli glycosides and other 4 components in Xianx-ionggukang particles show good linear relationship with the injection volume(r＞0.999);the average spiked recoveries are 98.9％-102.4％(RSD＜3.0％,n=6).The limits of content of icariin,epimedin C,baohuoside Ⅰ,and ferulic acid in the 3 batches of samples are 3.852,3.919,0.355 3,and 0.935 8 mg·g-1,respectively.Conclusion The method is simple and rap-id,with accurate and reliable results and good method reproducibility,which can be used for the quality control of Xianxionggu-kang particles and provide a basis for the development of draft quality standards.
关键词
仙芎骨康颗粒/淫羊藿苷/朝藿定C/宝藿苷Ⅰ/阿魏酸/HPLC含量测定Key words
Xianxionggukang particles/Icariin/epimedin C/Baohuoside Ⅰ/Ferulic acid/Determination of content引用本文复制引用
出版年
2024
NETL
NSTL
万方数据