Simultaneous Determination of Icariin,Epimedin C,Baohuoside Ⅰ and Ferulic Acid From Xianxionggukang Particles by HPLC-DAD
Objective To establish the simultaneous determination of icariin,epimedin C,baohuoside Ⅰ and ferulic acid from Xianxionggukang particles by HPLC-DAD.Methods A multi wavelength switching method was used,the chromatographic col-umn was Zorbax Eclipse SB-C18,with acetonitrile-1％glacial acetic acid water as the mobile phase gradient elution,flow rate 1.0 mL·min-1,column temperature 30 ℃.Results The peak areas of patchouli glycosides and other 4 components in Xianx-ionggukang particles show good linear relationship with the injection volume(r＞0.999);the average spiked recoveries are 98.9％-102.4％(RSD＜3.0％,n=6).The limits of content of icariin,epimedin C,baohuoside Ⅰ,and ferulic acid in the 3 batches of samples are 3.852,3.919,0.355 3,and 0.935 8 mg·g-1,respectively.Conclusion The method is simple and rap-id,with accurate and reliable results and good method reproducibility,which can be used for the quality control of Xianxionggu-kang particles and provide a basis for the development of draft quality standards.
Xianxionggukang particlesIcariinepimedin CBaohuoside ⅠFerulic acidDetermination of content
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