Study on the effect of Jianpi Huazhuo Recipe on atherosclerosis mice based on fecal meta-bonomics
Objective Based on liquid chromatography-mass spectrometry(LC-MS),the regulatory mechanism of Jianpi Huazhuo Recipe on fecal metabolites in atherosclerotic(AS)mice was studied.Methods The AS animal model was established by high choline diet,and the pathological changes of aortic plaque in mice were observed.The metabolic profile of mouse feces samples was analyzed by LC-MS technology,and the differential metabolites and metabolic pathways in mouse feces were identi-fied and screened by means of principal component analysis(PCA)and orthogonal least squares discriminant analysis(OPLS-DA).Results Jianpi Huazhuo Recipe can inhibit the formation of AS plaque in mice,regulate the serum lipid level,and recall 77 potential biomarkers,10 key biomarkers and 8 metabolic pathways related to AS(vitamin B6 metabolism,thiamine metabo-lism,steroid biosynthesis,glycerophosphate metabolism,purine metabolism,arginine and proline metabolism,primary bile acid biosynthesis and steroid hormone biosynthesis).Conclusion The mechanism of Jianpi Huazhuo Recipe in treating AS may be through the regulation of vitamin metabolism,nucleotide metabolism,lipid metabolism and amino acid metabolism in AS mice.
NETL
NSTL
万方数据
