Effect of Huangqin Decoction regulating miR-143/TLR2/NF-κB/NLRP3 pathway on scorch death in rats with damp-heat ulcerative colitis
Objective To explore the regulation of miR-143/TLR2/NF-κB/NLRP3 pathway by Huangqin Decoction on damp-heat ulcerative colitis rats.Methods The damp-heat model of ulcerative colitis was established by DSS+complex factors.The experiment was divided into normal group,model group,low-dose Huangqin Decoction group,medium-dose Huangqin de-coction group and high-dose Huangqin decoction group.The pathological changes were observed by HE staining,the expression of serum IL-1 β and IL-18 factor was detected by ELISA,and the expression levels of TLR2,NF-κB(p-p65)and GSDMD protein were detected by Western blot.mRNA levels of miR-143,TLR2,NLRP3,NF-κB,Caspase-1 and GSDMD in colon tissues were detected by qPCR.Results Compared with the normal group,the pathological tissue of colon in the model group was damaged,the levels of IL-1 β,IL-18 inflammatory factors,the protein expressions of TLR2,p-p65 and GSDMD were signifi-cantly increased,and the mRNA levels of NLRP3,GSDMD,TLR2,NF-κB and caspase-1 were significantly increased.miR-143 decreased significantly.Compared with the model,the colonic pathological tissue damage was improved in the medium and high dose Huangqin Decoction group,and the serum IL-1 β and IL-18 levels in the low dose group and the medium and high dose group were significantly reduced.The expression of p-p65 protein decreased significantly in low-dose group,and the ex-pression of TLR2,p-p65 and GSDMD protein decreased significantly in medium-high dose group.The mRNA expressions of NF-κB and NLRP3 were significantly decreased in the low-dose group.The mRNA levels of TLR2,NF-κB,caspase-1 and GSDMD were significantly decreased in the medium dose group.In high-dose group,miR-143 was significantly increased,and TLR2,NF-κB,GSDMD,NLRP3 mRNA were significantly decreased.Conclusion Huangqin Decoction may inhibit pyroptosis in rats with damp-heat ulcerative colitis by regulating miR-143/TLR2/NF-κB/NLRP3 pathway,so as to play a role on damp-heat UC.
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