苓桂术甘汤干预FXR途径影响游离脂肪酸诱导的HepG2细胞脂质代谢
Effect of Linggui Zhugan decoction on FXR pathway in free fatty acid-induced adipose accumulation model of HepG2 cells
张傲 1李然 1刘立萍1
作者信息
- 1. 辽宁中医药大学,辽宁沈阳 110847
- 折叠
摘要
目的 观察苓桂术甘汤对FFA诱导的HepG2细胞脂质代谢的影响,探讨其作用机制是否与其干预FXR途径有关.方法 选用HepG2细胞,采用1mmol/LFFA诱导,实验分为正常组、模型组、苓桂术甘汤组、阿托伐他汀组.采用CCK-8法测定细胞活力,油红O染色法观察脂滴分布,生物试剂盒测定TG、TC含量,蛋白免疫印迹法检测FXR、SHP、CYP7A1、LXR、SREBP-1蛋白的表达量,免疫荧光法检测FXR的荧光表达强度.结果 与正常组相比,模型组脂滴分布增加,TG、TC含量升高(P＜0.01),FXR、SHP蛋白表达水平降低,CYP7A1、LXR和SREBP-1蛋白表达水平升高,FXR荧光表达水平降低;与模型组比较,苓桂术甘汤组和阿托伐他汀组脂滴分布下降,TG、TC含量降低(P＜0.01),FXR、SHP蛋白表达水平升高,CYP7A1、LXR和SREBP-1蛋白表达水平降低,FXR荧光表达水平升高.结论 苓桂术甘汤可显著抑制游离脂肪酸诱导的HepG2细胞脂肪沉积,其作用机制可能与干预FXR介导的脂质代谢通路有关.
Abstract
Objective To observe the effect of Linggui Zhugan decoction on the lipid metabolism of HepG2 cells by free fatty acid-induced,and to investigate whether the mechanism of intervention with FXR.Methods HepG2 cells were selected and induced by 1mmol/L FFA,and the experiments were divided into normal group and model group and Linggui Zhugan decoction group and atorvastatin group.Cell viability was determined by CCK-8;the distribution of lipid droplets was observed by oil red O staining;cellular TG and TC content by biological kit;cellular FXR,SHP,CYP7A1,LXR and SREBP-1 protein expression by Western blot;FXR by immunofluorescence.Results Compared with the normal group,in the model group,lipid droplet distribution in-creased,TG and TC content increased(P＜0.01),FXR and SHP protein expression levels were decreased,higher protein ex-pression levels of CYP7A1,LXR and SREBP-1,FXR fluorescence expression levels were decreased.Compared with the model group,lower lipid droplet distribution in the Linggui Zhugan decoction and atorvastatin groups,TG and TC content was decreased(P＜0.01),FXR and SHP protein expression levels were increased,the protein expression levels of CYP7A1,LXR and SREBP-1 were decreased,FXR fluorescence expression levels were increased.Conclusion Linggui Zhugan decoction significantly in-hibited on the lipid metabolism of HepG2 cells by free fatty acid-induced,the mechanism of action may be related to the inter-vention of FXR mediated lipid metabolism pathway.
关键词
苓桂术甘汤/非酒精性脂肪肝病/脂质代谢/法尼醇X受体Key words
Linggui Zhugan decoction/Non-alcoholic fatty liver disease/Lipid metabolism/Farnesol X receptor(FXR)引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据