Effect of Linggui Zhugan decoction on FXR pathway in free fatty acid-induced adipose accumulation model of HepG2 cells
Objective To observe the effect of Linggui Zhugan decoction on the lipid metabolism of HepG2 cells by free fatty acid-induced,and to investigate whether the mechanism of intervention with FXR.Methods HepG2 cells were selected and induced by 1mmol/L FFA,and the experiments were divided into normal group and model group and Linggui Zhugan decoction group and atorvastatin group.Cell viability was determined by CCK-8;the distribution of lipid droplets was observed by oil red O staining;cellular TG and TC content by biological kit;cellular FXR,SHP,CYP7A1,LXR and SREBP-1 protein expression by Western blot;FXR by immunofluorescence.Results Compared with the normal group,in the model group,lipid droplet distribution in-creased,TG and TC content increased(P＜0.01),FXR and SHP protein expression levels were decreased,higher protein ex-pression levels of CYP7A1,LXR and SREBP-1,FXR fluorescence expression levels were decreased.Compared with the model group,lower lipid droplet distribution in the Linggui Zhugan decoction and atorvastatin groups,TG and TC content was decreased(P＜0.01),FXR and SHP protein expression levels were increased,the protein expression levels of CYP7A1,LXR and SREBP-1 were decreased,FXR fluorescence expression levels were increased.Conclusion Linggui Zhugan decoction significantly in-hibited on the lipid metabolism of HepG2 cells by free fatty acid-induced,the mechanism of action may be related to the inter-vention of FXR mediated lipid metabolism pathway.
Linggui Zhugan decoctionNon-alcoholic fatty liver diseaseLipid metabolismFarnesol X receptor(FXR)
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