Composition analysis of Pinelliae Rhizoma before and after processing with Zingiberis Rhizoma Recens based on HPLC-Q-TOF-MS/MS
Objective To identify the components of Pinelliae Rhizoma and Pinelliae Rhizoma Praeparatum cum Zingibere et Alu-mine by high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(HPLC-Q-TOF-MS/MS),and to find the the differences of chemical components before and after the processing with Zingiberis Rhizoma Re-cens.Methods The components of Pinelliae Rhizoma and Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine were detected by HPLC-Q-TOF-MS/MS technology.The collected sample data were imported into the mass spectrometry data analysis soft-ware Agilent MassHunter Qualitative Analysis 10.0,and each mass spectrum peak was extracted.The chemical composition was preliminarily identified by searching the self-built database.The composition was determined by combining the fragment ion in-formation and retention time of reference substances and related ingredients in the literature and MassBank database.In addition,the mass spectrometry data of Pinelliae Rhizoma and Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine were imported into Agilent Profinder 10.0 software,and the data were processed by peak extraction,alignment and normalization.Principal compo-nent analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were performed on the data using Simca 14.1 software.Results A total of 43 components were identified in Pinelliae Rhizoma and Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine,including 5 alkaloids,7 fatty acid glycerides,7 lysophosphatidylcholines(LPCs),5 alcohol amines,7 amino acids,5 amides,1 organic acid,2 gingerols and 4 other components.The results of PCA analysis showed that the chemi-cal components of Pinelliae Rhizoma before and after Zingiberis Rhizoma Recens processing were significantly different.The re-sults of OPLS-DA showed that 18 main differential components such as adenosine,guanosine and funtuminebase were screened by VIP value＞1 and|P(corr)|＞0.5.Conclusion There are obvious differences in some alkaloids,amides,gingerols and other compounds before and after Pinelliae Rhizoma processing.These differences may lead to the different emphasis on the effica-cy of Pinelliae Rhizoma before and after Zingiberis Rhizoma Recens processing,and provide a reference for improving the quality standards of Pinelliae Rhizoma and its processing products.
Pinelliae RhizomaPinelliae Rhizoma Praeparatum cum Zingibere et AlumineHPLC-Q-TOF-MS/MSPrincipal component analysisOrthogonal partial least square method
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