光谱法分析人参皂苷Rb3与牛血清白蛋白的相互作用
Spectral Analysis of the Interaction between Ginsenosides Rb3 and Bovine Serum Albu-min
李德慧 1屈飞勇 1黄意情 1杜雨华 1杨波 1苗艳萍 1田健 1许佳明 1林喆1
作者信息
- 1. 长春中医药大学药学院,吉林长春 130117
- 折叠
摘要
目的 研究人参皂苷Rb3(GSRb3)与牛血清白蛋白(BSA)的分子作用机制.方法 在模拟生理条件下,应用荧光光谱、吸收光谱和拉曼光谱测定了 GSRb3与BSA的相互作用机理、主要作用力类型、热力学参数和作用位点等.结果 GSRb3引起BSA荧光淬灭的机制为联合淬灭.热力学分析的结果表明范德华力和氢键是GSRb3和BSA分子之间的主要作用力.拉曼光谱结果表明GSRb3与BSA的色氨酸残基结合.结论 GSRb3与BSA之间相互作用形成复合物,该研究为进一步理解GSRb3与生物大分子的相互作用提供理论基础.
Abstract
Objective To study the interaction between ginsenoside Rb3(GSRb3)with bovine serum albumin(BSA)at the mo-lecular level.Methods Under simulated physiological conditions,the interaction mechanism,main force types and thermodynam-ic parameters of GSRb3 and BSA were determined by fluorescence,absorption and Raman spectroscopy.Results GSRb3 induced fluorescence quenching of BSA by a mixed quenching.The results of thermodynamic analysis indicated that van der Waals force and hydrogen bonding were the main forces of the complex.Raman spectroscopy results indicated that GSRb3 binds to tryptophan residues of BSA.Conclusion GSRb3 interacts with BSA to form a complex.This work provides a theoretical basis for further un-derstanding of the interaction between GSRb3 and biomolecules.
关键词
人参皂苷Rb3/牛血清白蛋白/相互作用/光谱法Key words
Ginsenosides Rb3/Bovine serum albumin/Interaction/Spectroscopy引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据