摘要
目的 观察电针"风府""太冲"穴对帕金森病(PD)小鼠代谢性谷氨酸5(mGluR5)及其通路的影响,探讨电针治疗PD的可能机制.方法 C57BL/6小鼠随机分为正常组(Ctrl)、模型组(MPTP)、抑制剂组(CHPG)、抑制剂加电针组(CHPG+EA),电针组(EA组),每组10只.用MPTP连续腹腔注射7天复制PD模型.电针"风府""太冲"穴,30 min/次,1次/d,连续治疗12天.观察各组小鼠行为学变化;免疫组化法检测mGluR5表达;Western blot法检测黑质中mGluR5、p-ERK、p-CREB、BDNF 蛋白表达,PCR 法检测 mGluR5、ERK、CREB、BDNF mRNA 表达水平.结果 与 Ctrl 组相比,MPTP组、CHPG组、CHPG+EA组小鼠出现行为学改变(P＜0.01),而EA组小鼠行为学有所改善(P＜0.01).MPTP组、CHPG组、CHPG+EA组小鼠黑质mGluR5阳性表达上升(P＜0.01),EA组小鼠黑质mGluR5阳性表达下降(P＜0.01).MPTP组、CHPG组、CHPG+EA组mGluR5的mRNA相对表达量及蛋白表达上升(P＜0.01),EA组mGluR5的mRNA相对表达量及蛋白表达下调(P＜0.01).MPTP组、CHPG组、CHPG+EA组ERK1/2、CREB和BDNF的mRNA相对表达量及蛋白表达下调(P＜0.01),EA组ERK1/2、CREB和BDNF的mRNA相对表达量及蛋白表达上调(P＜0.01).结论 电针"风府""太冲"穴可以改善PD小鼠的运动功能障碍,同时抑制中脑黑质mGluR5通路的激活.
Abstract
Objective To observe the effects of electroacupuncture at Fengfu and Taichong points on metabolic glutamate 5(mGluR5)and its pathway in mice with Parkinson's disease(PD),and to explore the possible mechanism of electroacupuncture in the treatment of PD.Methods C57BL/6 mice were randomly divided into normal group(Ctrl),model group(MPTP),inhibi-tor group(CHPG),inhibitor plus electroacupuncture group(CHPG+EA),and electroacupuncture group(EA group),with 10 mice in each group.The PD model was replicated by continuous intraperitoneal injection of MPTP for 7 days.Electroacupuncture was applied to Fengfu and Taichong points for 30 min/d once/d for 12 consecutive days.Behavioral changes of mice in each group were observed.mGluR5 expression was detected by immunohistochemistry;mGluR5,p-ERK,p-CREB and BDNF protein ex-pression in substantia nigra was detected by Western blot,and mRNA expression levels of mGluR5,ERK,CREB and BDNF were detected by PCR.Results Compared with the Ctrl group,mice in the MPTP,CHPG,and CHPG+EA groups showed behavioral changes(P＜0.01),while mice in the EA group showed improved behavior(P＜0.01).nigral mGluR5-positive expression increased in mice in the MPTP,CHPG,and CHPG+EA groups(P＜0.01),while nigral mGluR5 positive expression decreased(P＜0.01).mRNA relative expression and protein expression of mGluR5 in MPTP,CHPG,and CHPG+EA groups increased(P＜0.01),and mRNA relative expression and protein expression of mGluR5 in EA group was down-regulated(P＜0.01).ERK1/2 in MPTP,CHPG,and CHPG+EA groups,CREB and BDNF were down-regulated(P＜0.01),and the relative mR-NA expression and protein expression of ERK1/2,CREB and BDNF were up-regulated(P＜0.01)in the EA group.Conclu-sion Electroacupuncture at Fengfu and Taichong points improved the motor dysfunction of PD mice and inhibited the activation of mGluR5 pathway in the substantia nigra of the midbrain.
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