Regulatory effect of Sishen Wan on the balance between memory Treg and memory Th17 cells in colitis mice
Objective To explore the effect of Sishen Wan on the levels of memory Treg and memory Th17 cells in mice with coli-tis.Methods Forty SPF grade male BALB/c mice were randomly divided into four groups:normal group,normal+Sishen Wan group,model group,and model+Sishen Wan group.The model group and the model+Sishen Wan group were induced to es-tablish a colitis model using the DSS method.After drinking a 3％(W/V)DSS solution prepared with distilled water for 7 days,they drank distilled water for 7 days,and then drank a 2.5％(W/V)DSS solution for 7 days;The normal group and the normal+Sishen Wan group drank distilled water throughout the experiment.On the 7th day of the experiment,the normal+Sishen Wan group and the model+Sishen Wan group began to receive a 5g/kg dose of Sishen Wan suspension by gavage.The normal and model groups were given equal volume of drinking water by gavage.The therapeutic effect was evaluated using Disease Activi-ty Index(DAI),Colon Length,Colon Weight Index,Colon Weight to Length Ratio,and Pathological Injury Score.Flow cytome-try was used to detect the levels of central memory Treg and Th17 cells and effector memory Treg and Th17 cells in mouse mesen-teric lymph nodes,and mTreg/mTh17 was calculated.Results Compared with the model group,the model+Sishen Wan group showed a significant decrease in DAI score(P＜0.01),a significant increase in colon length(P＜0.01),a significant decrease in colon weight,colon weight to length ratio,and colon weight index(P＜0.01),and a significant decrease in colon pathological injury score(P＜0.01);The levels of central and effector memory Treg cells increased,while the levels of memory Th17 cells decreased.The ratio of central memory Treg to Th17 cells and the ratio of effector memory Treg to Th17 cells significantly in-creased(P＜0.01).Conclusion The effective treatment of DSS induced colitis in mice with Si Shen Wan may be related to the regulation of memory Treg/Th17 cell balance.
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