Exploring the mechanism of action of licorice in alleviating cisplatin liver injury based on LC-MS/MS metabolomics technology
Objective In order to study the mechanism of licorice's mitigating effect on cisplatin hepatic injury.Methods 48 SD rats were randomly divided into the blank group,the model group,the positive control group,and the licorice group(450,900,and 1800 mg/kg).Thirty minutes after administration on day 5,cisplatin(8 mg/kg)was intraperitoneally injected into the mod-el,positive control and licorice groups to establish an acute liver injury model.The metabolites and metabolic pathways of licorice to alleviate cisplatin acute liver injury were investigated using LC-MS/MS untargeted metabolomics.Results The clustering of metabolomics samples showed significant separation in principal component analysis plots,and the three groups in the OPLS-DA model could be clearly distinguished in multivariate statistical analysis.Of the 976 total metabolites,252 metabolites differed sig-nificantly between the blank and model groups,and licorice was able to modulate 33 of these differential metabolites.The differ-ential metabolites were imported into the MetaboAnalyst 5.0 database for metabolic pathway analysis,which revealed that the dif-ferential metabolites were mainly involved in metabolic pathways such as Starch and sucrose metabolism;Galactose metabolism;Phenylalanine,tyrosine and tryptophan biosynthesis;Tyrosine metabolism;cAMP signaling pathway;Valine,leucine and isoleu-cine biosynthesis;Valine,leucine and isoleucine degradation;Amino sugar and nucleotide sugar metabolism.Conclusion The results suggest that the mitigating effect of licorice on cisplatin liver injury may be closely related to its improvement in the levels of differential metabolites and regulation of amino acid metabolism and glucose metabolism and other related pathways.
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