Exploring the mechanism of Danzhi Jiangtang Capsule on diabetic kidney disease in rats based on TLRs/MyD88 signaling pathway
Objective To investigate the interventional effect and mechanism of Danzhi Jiangtang Capsule on renal injury in dia-betic nephropathy rats based on Toll-like receptors(TLRs)/myeloid differentiation factor 88(MyD88)pathway.Methods Six-ty SD rats were randomly divided into a blank group,a model group,a valsartan group,and a Danzhi Jiangtang Capsule group.After 16 weeks of intervention,changes in the general condition of the rats in each group were observed.Biochemical methods were used to measure glucose and lipid metabolism,and renal function-related indicators.Inflammatory factor levels were detec-ted by enzyme-linked immunosorbent assay.TLR2,TLR4,and MyD88 mRNA expression levels were detected by real-time fluorescent quantitative polymerase chain reaction.The expression levels of autophagy-related factors Beclin1,LC3-Ⅰ,and LC3-Ⅱ were detected by Western blot.Renal tissue cells of rats were observed by transmission electron microscopy.Reactive oxygen species levels in renal podocytes were detected by flow cytometry.Cell proliferation was measured by cell counting.Results Com-pared with the model group,rats in the Danzhi Jiangtang Capsule group and the valsartan group showed improved quality of life,significant improvement in glucose and lipid metabolism,and renal function-related indicators(P＜0.01).Levels of IL-1 βand TNF-α were reduced,while IL-10 levels were elevated(P＜0.05).TLR2,TLR4,and MyD88 mRNA expression in re-nal tissue cells increased(P＜0.05),while Beclin1,LC3-Ⅰ,and LC3-Ⅱ protein expression levels decreased(P＜0.05).Renal tissue pathology improved to varying degrees,oxidative stress levels decreased,and cell proliferation increased(P＜0.05).Conclusion Danzhi Jiangtang Capsule can improve the quality of life of diabetic nephropathy rats,reduce excessive auto-phagy of cells,and attenuate renal cell injury.Its mechanism of action may be related to the regulation of the TLRs/MyD88 signa-ling pathway.
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