Xiao'er Dingchuan Granule Improves Th17/Treg Immune Imbalance and Alleviates Air-way Inflammation Induced by RSV Infection in Asthmatic Mice
Objective To explore the effect and mechanism of the optimal extraction process of Xiao'er Dingchuan Granules on re-spiratory syncytial virus(RSV)induced inflammatory response in lung tissue of asthmatic mice.Methods Sixty SPF grade male Balb/c mice were randomly divided into a normal group,a model group,a high-dose group of Xiao'er Dingchuan Granules,a medium dose group of Xiao'er Dingchuan Granules,a low-dose group of Xiao'er Dingchuan Granules,and a positive drug(dexa-methasone)control group,with 10 mice in each group.Except for the normal group,other groups were challenged with respirato-ry syncytial virus and ovalbumin(OVA)aerosol inhalation to induce asthma.After successful modeling,the medication was ad-ministered continuously for two weeks.HE staining method was used to observe the pathological changes of mouse lung tissue;Observing the deposition of collagen and mucus reserve in the airway using Masson and periodic acid Schiff staining(PAS);ELISA method for detecting IL-6,lL-10,IL-17,and TGF-β in lung tissue and serum.Cytokine content;Western Blot de-tection of STAT3,p-STAT3,RORγt,Foxp3 protein expression level,qRT-PCR detection of RORγt,Foxp3mRNA expression.Results The results showed that HE staining showed changes in the airway structure of the model group mice,with an increase in inflammatory secretions.Masson and PAS staining showed a significant increase in airway mucus and collagen deposition in the model group;Compared with the model group,the airway structure of mice in each dose group of Xiao'er Dingchuan Granule and dexamethasone group gradually recovered,and the mucus reserve and collagen deposition improved;Compared with the blank group,the model group showed IL-6,IL-17,and TGF-β1 levels in lung tissue and serum.The content of cytokines signifi-cantly increased,while the content of IL-10 decreased(P＜0.01).The levels of IL-6,IL-17,and TGF-β in each dose group of Xiao'er Dingchuan Granule and dexamethasone groups were significantly increased.The expression of cytokines decreased and the expression of IL-10 increased(P＜0.01);Compared with the control group,the model group showed p-STAT3 and ROR γt significantly increased,while Foxp3 protein expression significantly decreased(P＜0.01);Compared with the blank group,the model group has a ROR γt.Foxp3mRNA expression significantly increased(P＜0.01);Compared with the model group,the ROR γt of each dose group of Xiao'er Dingchuan Granule and dexamethasone group.The expression of Foxp3mRNA was significantly reduced(P＜0.01).Conclusion Xiao'er Dingchuan Granule improves Th17/Treg immune imbalance inhibition and reduces IL-6,IL-10,IL-17,and TGF-β in serum and lung tissue,it reduces the migration of inflammatory cells,air-way inflammatory infiltration,and collagen deposition and alleviates RSV induced airway inflammatory response in asthma mice.
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