Effects of Musk containing serum on the migration of bone marrow mesenchymal stem cells mediated by MAPK signaling pathway
Objective To investigate the mechanism of Musk promoting the migration of bone marrow mesenchymal stem cells(BMSCs)in vitro.Methods Sixty SD rats were randomly divided into high,medium and low dose group and blank control group.the saline serum and serum of Musk were prepared.Fifteen SD rats,BMSCs were isolated from rats and cultured for the third generation,BMSCs were i-dentified by morphological,phenotypic identification,osteogenesis and adipogenic induction,after the identification of success,BMSCs were treated with Musk serum to detect cell proliferation rate,the effect of Musk on migration of BMSCs was detected by Transwell assay after the addition of MAPK signal pathway blocker.Results The rat BMSCs were spindle shaped adherent growth,arranged regularly and the cell morphology is consistent;phenotype identification:The expression of CD44 and CD90 was negative,and the expression of CD44 and CD34 was negative;the cells can differentiate into osteoblasts and adipocytes;compared with the control group,different concentra-tion of Musk group can improve the proliferation rate of BMSCs at different time paragraph(P＜0.05);compared with the control group,low concentration of Musk group increased the number of Musk BMSCs migration in different time(P＜0.05),the migration rate of low concentration Musk+PD treatment group was decreased with the prolongation of time,there was no significant difference between groups in different time periods(P＞0.05).Conclusion Musk can promote the migration of exogenous BMSCs in rats,the mechanism may be related to the activation of MAPK/ERK1/2 signaling pathways.
万方数据
维普
