MEBO Affects the EMT Process of Diabetic Ulcer Rat's Wounds through PI3K/AKT/FoxO1 Pathway
Objective To explore the promoting effect of MEBO on the process of wound re-epithelization in diabetes rats and its mech-anism.Methods 90 male Wistar rats were randomly divided into five groups:MEBO group,rb-bFGF group,model group,control group,blank group and 18 rats in each group.The control group established an acute wound model.The MEBO group,the rb-bFGF group,and the model group established a diabetes wound model.The control group and the model group rats were treated with external application of normal saline.The MEBO group and the rb-bFGF group were treated with external application of MEBO and rb-bFGF re-spectively.Blank group only performs Hair Removal Treatment.Six rats were randomly selected from each group on the 3rd,7th,and 14th days,and tissue samples of the wounds were collected and stored.Masson staining were performed to observe the morphological changes of wound tissue.Immunofluorescence,Western blotting,and qRT-PCR were used to detect the protein expressions of factors such as FoxO1,E-cadherin,and Vimentin in each group.Results On the 7th/14th day of modeling,the wound healing rate in the con-trol group,MEBO group,and rb-bFGF group was higher than that in the model group(P＜0.05),and the results of wound tissue stai-ning were better.On the 3th day of modeling,the expression levels of AKT protein in the MEBO group were higher than those in the model group(P＜0.05);On the 7th day,the expression levels of PI3K,AKT,FoxO1 and Vimentin protein in the MEBO and rb bFGF groups were higher than those in the model group(P＜0.05),while E-cadherin and claudin1 were lower than those in the model group(P＜0.05);On the 14th day,compared with the model group,the expression levels of AKT,FoxO1 and Vimentin protein in the MEBO group and rb-bFGF group decreased(P＜0.05),while the expression levels of E-Cadherin and Claudin1 increased(P＜0.05).Conclusion MEBO significantly promoted the healing of diabetic ulcer wounds,which was possibly achieved by activating PI3K/AKT/FoxO1 signaling pathway to affect the expressions of E-cadherin,Claudin1 and Vimentin in wound tissues,as well as the EMT process.
万方数据
维普
