Effect of Astragalus polysaccharide on serum inflammatory factors and thyroid tissue of auto-immune thyroiditis NOD.2h4 mice impact
Objective To observe the effects of Astragalus membranaceus polysaccharide(APS)on serum inflammatory factors,thyroid antibodies and thyroid histopathological morphology in autoimmune thyroiditis(AIT)NOD.2h4 mice.Methods A total of 90 6-8-week-old female NOD.H-2h4 mice with a weight of(25±5)g were randomly divided into 6 groups,including blank control group,model group,selenium preparation group and low-medium-and high dose Astragalus polysaccharide groups,with 15 mice in each group.The low-medium-and high-dose groups of Astragalus polysaccharides were given 200、400、and 800 mg·kg-1 of Astragalus polysaccha-rides per day,respectively,and the selenium preparation group was given 0.26 mg/kg-1 sodium selenite tablets per day,and the blank control group and the model agent group were given the same volume of distilled water per day for 8 weeks.The serum levels of interleu-kin-6(IL-6),interleukin-10(IL-10),interleukin-12(IL-12),interleukin-17(IL-17),transforming growth factor β1(TGF-β1),anti-thyroglobulin antibody(TgAb),thyroid-stimulating hormone(TSH)levels in the serum of mice in each group were detected.HE staining was used to observe the thyroid lymphocyte infiltration in each group.Results Compared with the blank con-trol group,the serum levels of IL-6,IL-12,IL-17 and TgAb were significantly increased in the model group,while those in the oth-er treatment groups were significantly decreased(P＜0.001).Compared with the blank control group,the serum levels of IL-10 and TGF-β1 in the model group decreased significantly,while those in the other treatment groups increased,and the difference was statisti-cally significant(P＜0.001).Compared with the blank control group,the serum TSH of mice in the other groups was increased,but there was no significant difference between the groups,which confirmed the non-hyperthyroidism and hypothyroidism.HE staining showed that the thyroid lymphocyte infiltration of mice in the AIT group was significant,and the thyroid lymphocyte infiltration in each treatment group was restored.Conclusion Astragalus polysaccharides may play a role in delaying the occurrence of AIT by regulating thyroid autoimmune function,inhibiting the release of pro-inflammatory cytokines,and reducing the degree of inflammatory infiltration of thyroid lymphocytes.
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