Evaluation of the antidepression efficacy of salidroside and its mechanism on hypothyroidism model rats
Objective To study the mechanism of anti-depression effect of salidroside on hypothyroidism rats.Methods The rats were randomly divided into normal group,model group,low-dose group,high-dose group and thyroxine group,with 5 in each group.Ex-cept the normal group,the other groups were given propyl thiouracil(PTU)to construct the hypothyroidism model.Since the model was established,the rats in thyroid group were given Euthyrox of 4.0µg/(kg·d),while the low-dose and high-dose groups were given salidroside of 15.0 and 30.0mg/(kg·d)respectively,with intragastric administration for 28 days.The behavioral assessment,inclu-ding forced swimming test and sucrose preference test and thyroid hormones,involving T3,T4 and TSH were measured before and after administration.The concentration of 5-HT in serum and inflammatory factors,concerning IL-6,IL-1βand TNF-αin brain tissue were detected after administration.Results Compared with the model group(negative control),the depression behavior of the rats in the low and high dose salidroside groups was significantly improved,that the forced swimming immobility time was significantly shortened(P＜0.05 or P＜0.01),while the sucrose preference was significantly increased(P＜0.01),the thyroid hormone level was significantly improved,that the serum concentrations of T3 and T4 were significantly increased(P＜0.05 or P＜0.01),while that of TSH was sig-nificantly decreased(P＜0.01),the serum concentrations of 5-HT was significantly increased(P＜0.05 or P＜0.01),and the in-flammatory injury of brain tissue was significantly alleviated,that the contents of Il-6,IL-1β and TNF-α in brain tissue were signifi-cantly decreased(P＜0.01).Compared with the thyroxine group(positive control),the high-dose salidroside group had no signifi-cant difference in improving thyroid hormone level and reducing inflammatory damage of brain tissue(P＞0.05),and significantly in-creased serum 5-HT concentration(P＜0.05).Conclusion The anti-depression effect of salidroside on hypothyroidism rats is a-chieved by improving thyroid hormone levels,reducing inflammatory damage of brain tissue,and increasing serum 5-HT concentration.
万方数据
维普
