Study on the Mechanism of P2X7-ICC Autophagy Balance in Visceral Hypersensitivity of Di-arrhea-Predominant Irritable Bowel Syndrome Rats Treated with Electroacupuncture
Objective To observe the effects of Electroacupuncture(EA)on visceral hypersensitivity and the expression levels of colon-ic P2X7 and autophagy-related proteins in a rat model of diarrhea-predominant irritable bowel syndrome(IBS-D),as well as to ex-plore the underlying mechanisms.Methods A total of 24 SD rats were randomly divided into a blank control group,a model group,and an electroacupuncture group,with 8 rats in each group,with eight rats in each group.The IBS-D model was induced by chronic unpre-dictable mild stress combined with intragastric administration of senna.Electroacupuncture was applied to"Zusanli"(ST36)and"Tian-shu"(ST25)alternately for 14 days.The visceral hypersensitivity was evaluated by visceral pain threshold,and the severity of diarrhea was evaluated using the Bristol stool form scale.The pathological changes of colon were observed by HE staining,and the ultrastructure of interstitial cells of Cajal(ICC)was observed by transmission electron microscope.The expression levels of IL-1β,TNF-α,C-kit,Beclin1,mTOR and P2X7 in colon tissue were detected by immunohistochemistry.Results Compared with the control group,the visceral pain threshold of the model group was significantly decreased(P＜0.01),and the Bristol score was significantly increased(P＜0.01).The mitochondria of colonic ICC cells were swollen,the rough endoplasmic reticulum was slightly dilated,and the autophago-somes were reduced.The positive expressions of IL-1β,TNF-α,C-kit,mTOR and P2X7 in colon tissue were significantly increased(P＜0.01,P＜0.01,P＜0.05,P＜0.01,P＜0.01),while the positive expression of Beclinl was significantly decreased(P＜0.01).Compared with the model group,the visceral pain threshold was increased(P＜0.05),and the Bristol score was decreased(P＜0.05)in the electroacupuncture group.The structure of colonic ICC cells was relatively normal,and obvious autophagosomes were ob-served.The positive expressions of IL-1β,TNF-α,C-kit,mTOR and P2X7 in colon tissue were significantly decreased(P＜0.05,P＜0.01,P＜0.05,P＜0.01,P＜0.01),while the positive expression of Beclin1 was increased(P＜0.05).Conclusion-Electroacupuncture can significantly alleviate visceral hypersensitivity and improve the diarrhea symptoms of IBS-D rats.Its mechanism may be related to reducing colonic P2X7 expression,promoting colonic ICC autophagy,and reducing the number of ICC.
AcupunctureDiarrhea-predominant irritable bowel syndromeVisceral hypersensitivityP2X7Interstitial cells of CajalAutophagy
万方数据
维普
