Effect of Different Concentration of LiQiHuaTanHuoXue Formula Containing Serum on Pro-liferation of Cardiac Microvascular Endothelial Cells in Rats
Objective To investigate the effects of LiQiHuaTanHuoXue Formula(LQHTHX)on the viability of hypoxia/reoxygenation-induced primary cardiac microvascular endothelial cells(CMECs)in rats.Methods The LQHTHX extract was prepared by boiling in water and concentrated in three doses of high(2.7 g of raw material/mL),medium(1.35 g of raw material/mL),and low(0.675 g of raw material/mL),and then gavaged into rats for 3 days at 10 mL/kg.Blood samples were taken at0.5h,1h,1.5h,2h,and 2.5 h after the last drug administration to isolate serum,which was prepared into 5％,10％,15％,and 20％drug-containing serum cell culture medium.The primary CMECs of neonatal rats were extracted and identified by immunofluorescence,and the cell hypoxia model was constructed in vitro.Different concentrations of drug-containing serum were added,and cell viability of each group was detected by the CCK8 method,comparing the differences in cell viability after treatment with different concentrations of drug-containing serum.Re-sults In this study,we successfully extracted rat primary CMECs and constructed a cellular hypoxia model.Compared with the model group,the positive drugs,nicorandil(200 μM)and valsartan(50 μM),as well as different concentrations of drug-containing serums in the middle-and high-dose groups could increase the viability of CMECs(P＜0.01),among which 10％and 15％LQHTHX-containing serums in the high-dose group had the best effect.Conclusion LQHTHX can effectively improve the hypoxia-induced de-crease in the viability of CMECs,thus exerting a protective effect on the endothelial function of cardiac microvessels.This study estab-lished the foundation for further in-depth investigation of the pharmacological mechanism of the cardioprotective effect of LQHTHX.
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