Effect of Jiawei Qinghao Biejia Decoction on CD34+cell-derived DC costimulatory molecules B7-1 and B7-2 in AML-CR patients
Objective To explore the possible mechanism of Jiawei Qinghao Biejia Decoction in treating Complete remission,CR)of a-cute myeloid leukemia,AML).Methods Twelve New Zealand white rabbits were randomly divided into normal group and high,middle and low dose groups of Jiawei Qinghao Biejia Decoction,with 3 rabbits in each group.The rabbits in the high,middle and low dosage groups of Jiawei Qinghao Biejia Decoction were given concentrated decoction of Jiawei Qinghao Biejia Decoction 30g,15g and 7.5g/(kg.d)by gavage for 3 days,while the rabbits in the normal group were given the same volume of distilled water by gavage,and blood was taken from the apex after the last administration to obtain normal rabbit serum and high,middle and low dosage rabbit serum of Jiawei Qinghao Biejia Decoction.The CD34+cells of AML-CR patients were isolated and purified by immunomagnetic beads method,and the CD34+cells were amplified.The amplified CD34+cells were divided into high,medium and low dose serum groups,normal serum groups,cytokine groups and blank groups.Except the blank group,all the other groups were induced to become dendritic cells,DC)by adding cytokines(GM-CSF+IL-4+IFN-α+TNF-α)for 9 days,and the cell morphology was observed by inverted microscope during the culture.The expression rates of DC surface antigen molecules CD80,CD86,CD83,CD1a and HLA-DR were detected by FCM,and the expressions of B7-1,B7-2 protein and mRNA in DC were detected by qRT-PCR and Western blot.Results Dendritic changes were never observed in DC cells in blank group.The cell bodies in the high,middle and low dose groups,cytokine group and normal serum group of Jiawei Qinghao Biejia Decoction were significantly enlarged,and stellate pleomorphism and dendritic processes ap-peared on the cell surface.Compared with the blank group,the expressions of DC surface markers B7-1,B7-2,CD83,CD1a and HLA-DR in the high,middle and low dose groups of Jiawei Qinghao Biejia Decoction increased significantly(P＜0.05).Compared with the cytokine group and the normal serum group,the expressions of DC surface markers B7-1,B7-2,CD1a and HLA-DR in the high,middle and low dose groups of Jiawei Qinghao Biejia Decoction were significantly increased(P＜0.05).The DC surface marker CD83 in the high and middle dosage groups of Jiawei Qinghao Biejia Decoction was significantly increased(P＜0.05).There was no sig-nificant difference in the relative expression of B7-1 and B7-2 mRNA and protein among blank group,cytokine group and normal ser-um group(P＞0.05).Compared with the blank group,cytokine group and normal serum group,the relative expressions of B7-1 and B7-2 mRNA and protein in high,medium and low dose serum groups of Jiawei Qinghao Biejia Decoction were significantly increased(P＜0.05).Conclusion The mechanism of Jiawei Qinghao Biejia Decoction in treating AML-CR may be related to its ability to effective-ly promote the maturation of CD34+cell-derived DC in AML-CR patients,thus increasing the expression of costimulatory molecules B7-1 and B7-2 in DC and enhancing the antigen presenting ability of DC.
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