Mechanism of apoptosis of synovial fibroblasts in human rheumatoid arthritis induced by FG-FR1 signaling from medicated serum of Juan Bi Qiang Gu Formula
Objective This study aims to explore the effects of Juan Bi Qiang Gu Formula-containing serum on the proliferation and ap-optosis of human rheumatoid arthritis synovial fibroblasts(RA-FLS)and its mechanism of action,focusing on the fibroblast growth fac-tor receptor 1 signaling pathway.Methods Human RA-FLS were treated with various concentrations of Juan Bi Qiang Gu Formula-containing serum.Cell proliferation was assessed using the CCK8 assay to determine the concentration at which the formula inhibits prolif-eration.LPS was used to induce an inflammatory response in RA-FLS,followed by intervention with the formula-containing serum.Flow cytometry was utilized to examine the apoptosis of RA-FLS cells.RT-PCR was conducted to measure the relative mRNA expres-sion levels,and Western blotting was employed to analyze the relative protein expression levels.Results The CCK8 assay revealed that the Juan Bi Qiang Gu Formula-containing serum significantly inhibited the proliferation of RA-FLS cells at 48h and 72h across various concentrations.Flow cytometry analysis indicated that,under LPS-induced inflammatory conditions,the formula-containing serum promoted both early and late apoptosis of RA-FLS cells.RT-PCR results showed that the formula downregulated the mRNA expression of anti-apoptotic genes AKT and Bcl-2 in RA-FLS cells induced by LPS.Western blot results demonstrated that the formula sup-pressed the phosphorylation levels of FGFR1 and the expression of the anti-apoptotic protein Bcl-2,while promoting the expression of the pro-apoptotic protein BAX in RA-FLS cells under LPS induction.Conclusion The Juan Bi Qiang Gu Formula-containing serum significantly inhibits the proliferation of LPS-induced RA-FLS cells and induces their apoptosis.The mechanism may be associated with the suppression of FGFR1 signaling pathway transmission.
Juan Bi Qiang Gu FormulaRheumatoid ArthritisSynovial FibroblastsCell ProliferationCell ApoptosisFibroblast Growth Factor Receptor 1
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维普
