Effects of Pingchuan Granules on M2 polarization,complement factor D expression and air-way remodeling of macrophages in asthmatic mice
Objective To observe the effects of Pingchuan granule on the M2 polarization of macrophage and the expression of comple-ment factor D in asthmatic mice,and to explore its possible mechanism of prevention and treatment of airway remodeling in asthma.Methods Forty female C57BL/6 mice were randomly divided into blank group,model group,budesonide group and Pingchuan granule group,with 10 mice in each group.Except for the blank group,the other three groups were modeled by intraperitoneal injection and Aerosol inhalation of OVA.After successful modeling,they were given corresponding medications,and the materials were taken after 4 weeks of treatment.The pathological changes of mice lung tissue were observed by Masson staining;ELISA was used to detect the levels of IL-4 in BALF and serum,Flow Cytometry was used to detect the expression levels of M1 macrophages(F4/80+CD86+)and M2 macrophages(F4/80+CD206+),Western blot method was used to detect the expression of FIZZ1,Ym1,CFD and α-SMA in lung tis-sue,and Immunofluorescence double staining was used to detect the expression of F4/80 and CFD in lung tissue.Results The experi-mental study showed that compared with the blank group,the model group showed a large amount of collagen fiber deposition,the level of IL-4 in BALF and serum was significantly increased,the percentage of polarization of M2 and M1 macrophages in lung tissue was signif-icantly increased,the expression of FIZZ1,Ym1,CFD and α-SMA protein in the lung tissues was significantly increased,and there was co-expression of F4/80 and CFD,and the fluorescence intensity of F4/80 and CFD was significantly enhanced(P＜0.01);com-pared with the model group,Pingchuan granule could reduce collagen fiber deposition,significantly reduce the level of IL-4 in BALF and serum,the percentage of the M2 polarization of macrophage in lung tissue,the expression of FIZZ1,Ym1,CFD and α-SMA pro-tein in lung tissues was also significantly reduced,and decreased fluorescence intensity of F4/80 and CFD(P＜0.01).Conclusion-Pingchuan granule may reduce the expression of CFD,thereby inhibiting the M2 polarization of macrophage,and finally achieve the pur-pose of treating asthma airway remodeling.
Pingchuan granuleAsthmaAirway remodelingM2 polarization of macrophageCFD
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