Study on the protective effect of Fuzi Ganjiang medicated serum on H9C2 cells against hypoxia reoxygenation injury
Objective Study the protective effect of Fuzi Ganjiang serum(FG)on H9C2 cells after hypoxia/reoxygenation(H/R)inju-ry.Methods FG medicated serum was prepared from rats,and H9C2 cells were used to establish H/R injury model in vitro.H9C2 cells were divided into Control group(Con),H/R group and FG group.The cell viability was detected by CCK-8 method,the apoptosis rate and ROS were detected by flow cytometry,the expressions of SOD,MDA,TNF-α and IL-10 were detected by kit,the gene and pro-tein expressions of TRPV1 and CGRP were detected by RT-PCR and WB,respectively.Results FG could improve the survival rate of H9C2 cells after H/R injury(P＜0.01),decreased the expression of MDA,TNF-α,IL-10 apoptosis,ROS.increased the expres-sion of SOD,and increased the gene and protein expression of TRPV1 and CGRP.Conclusion FG can improve the survival rate of H9C2 cells after H/R injury by activating TRPV1/CGRP signaling pathway,and reduce oxidative damage and inflammatory damage.
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