Ginseng and Polygonatum Medicine for Improving Mechanisms of Depression with Cognitive Impairment
Objective This study aimed to investigate the mechanisms by which a combination of Ginseng and Polygonatum(GP)im-proves cognitive impairment associated with depression by analyzing its effects on PI3K,ICAM-1,and MCP-1 expression in the hippo-campus of depressive rats.Methods Fifty male SD rats were randomly divided into blank control,model,Western medicine,high-dose GP,and low-dose GP groups.A rat model of depression was established using chronic unpredictable mild stress(CUMS)for 21 days.Rats in the Western medicine group were orally administered fluoxetine hydrochloride,while rats in the high-dose and low-dose GP groups received GP extract for 14 days.Open field test was used to assess depressive behavior,HE staining was employed to observe hip-pocampal cell morphology,enzyme-linked immunosorbent assay(ELISA)was conducted to measure hippocampal levels of DA and 5-HT,PCR was used to measure ICAM-1 mRNA levels in the hippocampus,immunohistochemistry was performed to measure NF-κB and MCP-1 protein expression levels,and Western blot(WB)was conducted to measure relative expression of PI3K protein in the hip-pocampus.Results After 14 days of treatment,compared with the model group,rats treated with GP showed significantly increased total distance moved and average speed in the open field test.Levels of DA and 5-HT in the hippocampus were elevated,relative expression of PI3K protein was significantly increased,protein expression levels of NF-κB and MCP-1 were significantly decreased,and ICAM-1 levels were reduced.GP treatment improved all these indicators in the treated groups compared to the model group.Conclusion GP treatment can improve depressive behavior and associated cognitive impairment in CUMS rats.The antidepressant effects of GP may be re-lated to its regulation of hippocampal pro-inflammatory cytokine release and expression.
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