Investigating the Therapeutic Effect of Atractylodis Macrocephalae-Paeonia Lactiflora Pairs on IBS-D Model Rats Based on the TLR4-NF-κB Pathway
Objective To explore the effects of Atractylodes macrocephala-Paeonia lactiflora drug pair on the targets related to TLR4/NF-κB signalling pathway in the model rats of diarrhoeal irritable bowel syndrome(IBS-D)in an attempt to elucidate the potential mechanism of its therapeutic effect on the treatment of low-grade inflammatory response.Methods IBS-D model rats were established by senna gavage+chronic unpredictable stimulation,and randomly divided into model group,drug-pair group,Atractylodes macro-cephala group,Paeonia lactiflora group,and western medicine group,with 6 rats in each group.Take another 6 rats without modeling and set as the blank group.The rats in each drug group were gavaged with the corresponding drug solution and treated continuously for 14 d.The intervention effects of Atractylodes macrocephala and Paeonia lactiflora sinensis on the symptoms of the model rats were evaluated by body weight,water content of stool,and AWR scores;blood was taken from the abdominal aorta of the rats in each group,and tissues of the terminal part of the ileum were dissected,and the intervention effect of Atractylodes macrocephala and Paeonia lactiflora sinensis on the symptoms of inflammation as well as TLR4/NF-κB signalling pathway of the IBS-D rats was detected by qRT-PCR,HE staining,Elisa,and WB methods.NF-κB signalling pathway related targets regulation.Results Compared with the model group,the drug pair improved body weight,reduced stool water content(P＜0.01),sugar-water preference(P＜0.01),reduced AWR scores at 40 mm-Hg and 60 mmHg pressures(P＜0.01),improved the inflammatory pathological state of ileocecal terminal tissues,down-regulated TLR4,NF-κB,and IL-1β mRNA expression(P＜0.01),down-regulated serum TLR4,NF-κB,MyD88 expression,up-regu-lated serum IL-10 expression,and down-regulated MyD88,p-NF-κB p65,IL-6 protein expression(P＜0.01).Conclusion The effect of Paeonia lactiflora on improving diarrhoea symptoms and visceral hypersensitivity in IBS-D rats may be related to the inhibition of the TLR4/NF-κB signalling pathway,which regulates the expression level of inflammatory response cytokines.
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