Actinomycete Community Structures and Diversities in Rhizosphere Soils in Different Growth Stages of Crocus sativus L.Analyzed Based on Illumina Novaseq high-throughput sequencing
Objective To study the structures and diversities of soil actinomycetic community in the rhizosphere soils of C.sativus L.at different growth stages.Methods The total DNA of rhizosphere soil Actinomycetes in different growth stages of C.sativus L.was extracted and sequenced using Illumina Novaseq high-throughput sequencing platform.Results A total of 1686 taxa(OTUs),including 15 phy-la,were obtained from three groups of samples from three growth stages of saffron(S1 at initial growth stage,S2 at middle growth stage and S3 at late growth stage)by high-throughput sequencing.The top 20 Actinomycetes with relative abundance were analyzed.Among them,the dominant actinomycete communities at Phyla level of saffron in different growth stages include Actinobacteria,Firmicutes,Pro-teobacteria and Bacteroidetes.And the dominant actinomycete communities at genus level of saffron in different growth stages are Rhodo-coccus,Blastococcus,Frankia,Agrococcus,Microbacterium and Conexibacter.The differences are that the dominant actinomycete communi-ties in S1 growth stage also include Nocardioides,Mycolicibacterium and Candidatus Microthrix,and S2 includes Arthrobacter,Mycolicibac-terium and Williamsia,S3 also includes Nocardioides,Solirubrobacter,Modestobacter,Mycobacterium,Glycomyces and Brachybacterium.The results of Alpha and Beta diversity analysis showed that the diversity of actinomycete communities in the rhizosphere soils of C.sati-vus L.at different growth stages are diverse.And the diversity of actinomycete community shows a rising trend with the growth of C.sati-vus L.Conclusion In this study,the rhizosphere growth promoting bacteria and rare actinomycete with antagonistic effect on pathogenic microorganisms,synthesis of secondary metabolites,and relative abundance in the rhizosphere soils of C.sativus L.can be detected.These results laid an important foundation for further study of its growth promoting function,stress resistance on saffron and antagonistic mechanism against pathogenic bacteria,viruses and other harmful microorganisms in the rhizosphere microenvironment,as well as the in-fluence of rhizosphere microbial interaction on seed ball quality of saffron bulb.
High-throughput sequencingCrocus sativus L.Rhizosphere soilActinomycete CommunityStructure and di-versity
NETL
NSTL
万方数据
