Study on the Jiawei Shaofu Zhuyu Decoction on IL-6/GP130 Pathway in Endometriosis Dysmenorrhea
Objective This study aims to explore the effects of Jiawei Shaofu Zhuyu Decoction on the symptoms of dysmenorrhea in mice with cold-dampness stasis and endometriosis,as well as its impact on the balance of T-helper 1(Th1)/T-helper 2(Th2)cells in the peritoneal fluid,based on the interleukin-6(IL-6)/Glycoprotein 130(GP130)signaling pathway.Methods The model of dys-menorrhea in mice with cold-dampness stasis and endometriosis was established using abdominal injection and ice water bath method.Hematoxylin-eosin(HE)staining and Masson trichrome staining were used to compare the pathological changes.Western blot was used to determine the protein expression levels of IL-6,IL-6R,and GP130.Flow cytometry was used to detect the number of Th1 and Th2 cells.ELISA was used to measure the levels of interleukin-4(IL-4),interleukin-10(IL-10),interleukin-12(IL-12),and interferon-γ(INF-γ)in the peripheral blood.Results After drug intervention,compared with the model group,the treatment groups showed prolonged pain latency,reduced twisting frequency,alleviated pelvic adhesion,decreased overall volume of ectopic lesions,and significant results in the high-dose herbal medicine group and positive drug group(P＜0.05).Pathological examination showed im-proved inflammatory response and reduced collagen fiber deposition after drug intervention.The expression of IL-6,IL-6R,and GP130 proteins in ectopic tissue decreased after drug intervention.The proportion of Th1 cells in the peritoneal fluid increased,while the proportion of Th2 cells decreased.The levels of IL-4 and IL-10 in the peripheral blood decreased,while the level of INF-γand IL-12 increased.Conclusion Jiawei Shaofu Zhuyu Decoction can improve the Th1/Th2 balance in the peritoneal fluid of mice with cold-dampness stasis and endometriosis,may be related to the regulation of the IL-6/GP130 signaling pathway,reduce the inflammatory state of the abdominal cavity and clinical symptoms in model mice.
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