Effects of Maxing Shigan Decoction on NLRP3 and IFITM3 in the lung tissues of Mice with In-fluenza Virus Pneumonia
Objective Study the effects of Maxing Shigan Decoction on the expression of NLRP3 and IFITM3 in the lung tissues of mice with influenza virus pneumonia,and explore the potential mechanisms for preventing and treating influenza virus infection.Methods-Randomly divide 72 male Balb/c mice into 4 groups:Maxing Shigan Decoction group,Oseltamivir group,model group,and blank group.The mice were intranasally infected with the influenza virus to establish the model.The treatment group was administered Maxing Shigan Decoction,while the control group was given Oseltamivir granules.On the 3rd and 7th days of treatment,9 mice from each group were selected for lung tissue collection.Western Blot and RT-PCR methods were used to detect the expression of NLRP3,IFITM3 pro-teins,and mRNA in the lung tissues.Results After 3 days of administration,compared with the control group,the mice in the model group showed signs of lung tissue congestion and oedema,accompanied by a large number of infiltrating lymphocytes and macrophages,significant damage to the alveolar structure,alveolar collapse or even disappearance,and a significant increase in the expression of NL-RP3 protein and mRNA in lung tissue(P＜0.01),while the expression of IFITM3 protein and mRNA decreased significantly(P＜0.01).Compared with the model group,Maxing Shigan Decoction and Oseltamivir could both regulate the changes in NLRP3 and IFITM3 in mouse lung tissue(P＜0.01 or P＜0.05).After 7 days of administration,the lung tissue structure in each drug group was basically normal,with a few inflammatory cells still infiltrating in the Oseltamivir group;the lung tissue morphology in the Maxing Shigan Decoction and the Oseltamivir group was similar to the control group,with clear alveolar contours.Compared with the control group,there was an increase in the expression of NLRP3 protein and mRNA in the lung tissue of the model group(P＜0.01).In comparison with the model group,Maxing Shigan Decoction could significantly reduce the content of NLRP3 in lung tissue(P＜0.01)while increas-ing the expression of IFITM3(P＜0.01).Compared with the Oseltamivir group,there were significant differences in the expression of NLRP3 and IFITM3 in the lung tissue of the Maxing Shigan Decoction group(P＜0.05).Conclusion Maxing Shigan Decoction may re-duce lung inflammation by inhibiting the expression of NLRP3 in the lung tissue of mice with influenza pneumonia,while upregulating the expression of IFITM3.Its effectiveness in alleviating lung inflammation is superior to oseltamivir and it offers a certain protective effect a-gainst lung damage caused by the influenza virus.
Maxing Shigan DecoctionInfluenza A virusPneumoniaNLRP3IFITM3
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