Effect of Jianpi Zishen formula on platelet cell apoptosis in MRL/Lpr lupus rats through PI3K/AKT pathway
Objective To investigate the effect of Jianpi Zishen formula on PI3K/AKT platelet apoptosis pathway in MRL/Lpr lupus rats.Methods C57BL/6 mice were taken as blank group,and MRL/lpr lupus mice were randomly divided into model group,Jianpi Zishen recipe low-dose group(6.32g/kg),Jianpi Zishen recipe high-dose group(12.64g/kg)and prednisone group(5mg/kg),with 6 mice in each group.Platelet count(PLT),anti-dsDNA,anti-nuclear antibody(ANA)and complement 3(C3)levels in pe-ripheral blood of mice were detected.The expression of related factors in PI3K/AKT pathway was detected by RT-qPCR.The expres-sions of PI3K/AKT pathway related factors and apoptosis factors Bax,Bel-2 and Caspase-3 were detected by Western blot.The apop-tosis of platelet cells was detected by flow cytometry.Results Compared with blank group,PLT,C3,Bel-2 mRNA and Bel-2 protein in model group were significantly decreased(P＜0.01).anti-dsDNA and ANA were significantly increased(P＜0.01).The relative expressions of PI3K mRNA,AKT mRNA and Bax mRNA were significantly increased(P＜0.01).The protein levels of P-PI3K,P-Akt,Bax,Caspase-3 and the apoptosis rate of platelet cells were significantly increased(P＜0.01).Compared with model group,PLT,C3,Bel-2 mRNA and Bel-2 were significantly increased in Jianpi Zishen recipe low-dose group,high-dose group and pred-nisone group(P＜0.01,P＜0.05),while anti-dsDNA and ANA were significantly decreased(P＜0.01).The relative mRNA ex-pression levels of PI3K,AKT and Bax were significantly decreased(P＜0.01),the protein expressions of P-PI3K,P-Akt,Bax and Caspase-3 were significantly decreased(P＜0.01),and the apoptosis rate of platelet was significantly decreased(P＜0.01).Con-clusion Jianpi Zishen formula may regulate the apoptosis of platelet cells through PI3K/AKT pathway,so as to ameliorate SLE thrombo-cytopenia.
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