Exploring the Mechanism of Qingjin Tongluo Granules in intervening Mycoplasma Pneumoni-ae Pneumonia Based on the Pathway of Cell Pyroptosis
Objective To explore the intervention mechanism of Qingjin Tongluo Granules on Mycoplasma Pneumonia Pneumonia(MPP)mice through NLRP3/Caspase-1/GSDMD cell pyroptosis pathway.Methods Sixty Balb/c mice were randomly divided into blank group,model group,Qingjin Tongluo Granule group,MCC950(NLRP3 inhibitor)group,Qingjin Tongluo Granule+MCC950 group,and Azithromycin group.A mouse model of MPP was established using the nasal drip method of Mycoplasma Pneumoniae(MP)strain.The rats were treated with drugs after modeling,and the materials were taken after 14 days of treatment.The pathological changes of mouse lung tissues were observed by HE staining.The contents of IL-1β and IL-18 were detected by ELISA.The protein expres-sions of NLRP3,caspase-1,ASC and GSDMD were detected by Western blot.The mRNA levels of NLRP3,caspase-1,ASC,GSD-MD,IL-1β and IL-18 in mouse lung tissues were detected by PCR.Results Compared with the blank group,the model group had pathological injury in lung tissue,increased IL-1 β and IL-18 contents in serum and lung tissue,increased NLRP3,caspase-1,ASC,GSDMD protein expression levels in lung tissue,and increased NLRP3,caspase-1,ASC,GSDMD,IL-1 β,IL-18 mRNA ex-pression in lung tissue(P＜0.05).Compared with the model group,the pathological injury of lung tissue in the other four groups was improved to varying degrees,the contents of IL-1 β and IL-18 in serum and lung tissue were decreased,the protein expression levels of NLRP3,caspase-1,ASC,GSDMD in lung tissue were decreased,and the mRNA expression levels of NLRP3,caspase-1,ASC,GSDMD,IL-1 β,IL-18 in lung tissue were decreased(P＜0.05).Conclusion Qingjin Tongluo Granules may improve lung inflam-mation in MPP mice by regulating NLRP3/Caspase-1/GSDMD mediated cell pyroptosis.
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