Based on miRNA-21a-5p/STAT3 axis,this paper discusses the effect of WuHuTang on asthma triggered by exosomes induced by RSV
Objective Based on the miRNA-21a-5p/STAT3 axis,to explore the effect of WuHuTang on asthma triggered by the exo-somes induced by RSV.Methods Forty SPF C57 mice were randomly divided into blank group,model group,WuHuTang group and ribavirin group.The blank group was given PBS nasal drops,and the rest groups were given PBS diluted with RSV-infected bone mar-row mesenchymal stem cell exosomes(BMSCs-EXO-RSV)nasal drops,once every other day for 7 times.Twenty-four hours after the intervention,the blank group and the model group were given distilled water by gavage,the WuHuTang group was given WuHuTang by gavage,and the ribavirin group was given ribavirin diluted aqueous solution by gavage for one week.During the intervention,the gen-eral behavior changes of mice in each group were observed,and the symptoms and signs of asthma were evaluated.After the intervention,the lung tissue of mice was taken for detection.HE and Masson staining were used to observe the inflammatory infiltration and collagen deposition in lung tissue,RT-QCPR method was used to detect the expression of miRNA-21 a-5p and STAT3 mRNA in lung tissue,Western blot method was used to detect the expression of STAT3 and p-STAT3 protein in lung tissue,and double luciferase experiment in vitro verified the targeted regulation of miRNA-21 and STAT3 mRNA.Compared with the blank group,the model group showed typ-ical changes of asthma,inflammatory infiltration and collagen deposition in lung tissue increased significantly,and the expression levels of miRNA-21a-5p,STAT3 mRNA and p-STAT3 in lung tissue increased(P＜0.01).Compared with the model group,the symp-toms and signs of WuhuTang group were improved,and the expression levels of miRNA-21a-5p,STAT3 mRNA and p-STAT3 in lung tissue were decreased(P＜0.01).The results of double luciferase in vitro showed that miRNA-21a-5p could promote the ex-pression of STAT3 mRNA(P＜0.05).Conclusion BMSCs-Exo-RSV can activate the miRNA-21a-5p/STAT3 axis,promote the expression of miRNA-21a-5p,STAT3mRNA andp-STAT3 protein in mouse lung tissue,cause airway inflammation and remodeling,and thus induce asthma,while WuHuTang can improve asthma by reversing the above links.
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