Effect of Dahuang Lingxian prescription on inhibiting biliary stone formation by regulating ex-osome LINC00311
Objective To investigate the mechanism of Dahuang Lingxian prescription in inhibiting stone formation and preventing chole-lithiasis by regulating exosome LINC00311.Methods The 70 male SD mice were randomly divided into blank control group,model group,ursodeoxycholic acid group,Dahuang Lingxian group,model+gene block group(model KO group),ursodeoxycholic acid+gene block group(ursodeoxycholic acid KO group),Dahuang Lingxian+gene block group(Dahuang Lingxian KO group),with 10 mice in each group.The mice model of cholelithiasis was induced by high cholesterol and high calorie lithogenic diet.The UNC00311 gene knockout mice model was established according to the in vivo transfection experiment.After 6 weeks of continuous intervention,the stone formation rate of mice in each group was recorded,the ultrastructural changes of cells in bile duct tissue were observed by electron micro-scope,the expressions of LINC00311 and TGF-β1/Smads signaling pathway were detected by RT-PCR,and the expression of metab-olites in bile was detected by 1H NMR.Results The stone formation rate of the Dahuang Lingxian group was 0％,which was lower than model group,model KO group,and Dahuang Lingxian KO group(P＜0.002).Electron microscopy showed that the structure of organ-elles in the Dahuang Lingxian group were complete,which was better than that in the model group,model KO group,and Dahuang Lingxian KO group.RT-PCR showed that LINC00311,Smad4 and Smad7 in Dahuang Lingxian group were significantly higher than those in model group,model KO group and Dahuang Lingxian KO group(P＜0.05),while TGF-β1,Smad2 and Smad3 were de-creased(P＜0.05).1H NMR showed that there were 9 metabolites in the bile of Dahuang Lingxian group with significant differences compared with model group.Conclusion Dahuang Lingxian prescription can promote the accumulation of exosome LINC00311 in the bili-ary system,improve cell structure,regulate inflammatory response and fibrosis,stabilize bile metabolism,prevent and treat cholelithia-sis.
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