Dynamic study on multiple components of Polygonatum cyrtonema
Objective Ultraviolet-visible spectrophotometry and High performance liquid chromatography(HPLC)were used to analyze the content change trend of multiple components in Polygonatum cyrtonema.Methods Agilent ZORBAX-Extend-C18(250 mm × 4.6 mm,5 μm)column was used with methanol-water as mobile phase for the determination of 5-Hydroxymethylfurfural,5-Hydroxy-maltol and polygonatine A.The content of fructose,glucose and sucrose were determined by chromatographic column Prevail carbohydrate ES(4.6 mm × 250 mm,5 μm)with acetonitrile-water as mobile phase.The contents of polysaccharides and saponins were deter-mined by UV-2450 UV-visible spectrophotometer.The alcohol-soluble extract was determined according to the 2020 edition of Chi-nese Pharmacopoeia'.The content changes of each component with the increase of steaming times were analyzed.Results With the in-crease of steaming times of Polygonatum cyrtonema,the contents of 5-Hydroxymethylfurfural,5-Hydroxymaltol and polygonatine A in-creased,the contents of polysaccharides,saponins,glucose and alcohol-soluble extract decreased,and the content of sucrose increased first and then decreased.Conclusion The number of steaming and drying times has a great influence on the content of each component.The content of each component of Polygonatum cyrtonema can be controlled by the processing technology of nine-steam-nine-bask,which provides a theoretical basis for the quality control of its processing process.
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