摘要
目的 比较"上巨虚"与"天枢"配伍"上巨虚"对IBS模型大鼠心率变异性及血清cAMP、cGMP、SP含量的影响,探讨"合募配穴"调节IBS大鼠自主神经平衡性是否存在协同效应.方法 选用雄性清洁级Wistar大鼠55只,随机分为空白组(10只)和造模组(45只),造模组采用急-慢性应激联合制备IBS模型,将制备成功IBS模型大鼠随机分为模型组(10只)、单穴组(10只)、合募配伍组(10只);单穴组、合募配伍组连续电针干预14 d;电针治疗后,记录各组大鼠体重、AWR半定量评分、行为学和心率变异性以及检测血清cAMP、cGMP、SP含量.结果 干预前,与空白组相比,模型组与电针治疗组大鼠体质量降低,AWR评分升高,大鼠内脏敏感性增加(P＜0.05、P＜0.01);治疗后,与模型组相比,电针能明显增加大鼠的体质量(P＜0.01),降低内脏敏感性(P＜0.05、P＜0.01),增加大鼠进入开放臂次数、停留时间以及活动轨迹的总路程(P＜0.05、P＜0.01),降低 LF/HF(P＜0.05),升高 HF 以及 RMSSD(P＜0.05),降低血清 cAMP、cGMP、SP 含量(P＜0.01、P＜0.05、P＜0.01);电针单穴组与合募配伍组相比,大鼠体质量、AWR评分、行为学、HRV以及血清cAMP、cGMP、SP含量均无统计学差异.结论 单穴以及合募配穴均能有效提高IBS内脏敏感性,缓解焦虑负性情绪,调节心率变异性,降低血清cAMP、cGMP、SP含量,但合募配穴并未表现出协同效应.
Abstract
Objective To compare the effects of"ST37"and"ST25"on heart rate variability and serum cAMP,cGMP and SP levels in rats with IBS model.We investigated whether there was a synergistic effect of the combined acupoints in regulating the autonomic balance of IBS rats.Methods 55 male clean-grade Wistar rats were selected and randomly divided into blank group(10 rats)and modelling group(45 rats),and the modelling group was prepared by acute and chronic combined IBS model,and the successfully prepared IBS model rats were randomly divided into the modelling group(10 rats),the single-point group(10 rats),and the combined-point group(10 rats);the single-point group and the combined-point group were continuously intervened by electroacupuncture for 14 days;after the electroacupuncture treatment,the rats in each group were recorded.After treatment,the body weight,semi-quantitative AWR score,behavioural and HRV measurements,and serum cAMP,cGMP and SP levels were recorded in each group.Results Before inter-vention,compared with the blank group,rats in the model group and the electroacupuncture treatment group had lower body mass,higher AWR scores,and increased visceral sensitivity(P＜0.05,P＜0.01);after treatment,compared with the model group,electroacupunc-ture significantly increased body mass(P＜0.01),decreased visceral sensitivity(P＜0.05,P＜0.01),increased the number of times the rats entered the open arm,the residence time,and total distance travelled by the activity trajectory(P＜0.05,P＜0.01),decreased LF/HF(P＜0.05)and elevated HF as well as RMSSD(P＜0.05),and decreased serum cAMP、cGMP and SP content(P＜0.01,P＜0.05,P＜0.01);there were no statistical differences in body mass,AWR score,behavioural,HRV,and serum cAMP,cGMP and SP content of the rats in the electroacupuncture single-point group compared with the combination and matching group.Conclusion Both the application of single acupoint and combined acupoints have been demonstrated to effectively enhance visceral sensitivity in IBS pa-tients,alleviate anxiety and negative affect,modulate heart rate variability,and decrease the serum levels of cAMP,cGMP,and sub-stance P.but the use of combined acupoints did not exhibit an additive synergistic effect.
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