基于数据挖掘探析吴献群治疗卵巢储备功能下降的用药规律及实验验证
Based on data mining to explore the law of traditional Chinese medicine for the decline of o-varian reserve
赵井苓 1萧闵 1江晓翠 1李成银 2王娟 3彭碧芳 4吴献群 5谢莉1
作者信息
- 1. 湖北中医药大学,湖北武汉 430065
- 2. 湖北中医药大学,湖北武汉 430065;湖北省中医院,湖北武汉 430061
- 3. 武警湖北总队医院,湖北武汉 430061
- 4. 武汉市东湖新技术开发区花山街社区卫生服务中心,湖北武汉 430070
- 5. 湖北省中医院,湖北武汉 430061
- 折叠
摘要
目的 探析吴献群治疗卵巢储备功能下降(DOR)的用药规律,并通过动物实验验证.方法 基于古今医案云平台构建数据库,收集2019年至2021年经吴献群诊治的DOR病案,进行用药规律进行分析并筛选基础方.在此基础上,复制DOR大鼠模型,给予基础方干预,通过阴道涂片观察模型大鼠排卵周期;伊红-苏木素(HE)染色评估卵巢功能;酶联免疫法(ELISA)检测血清抗弥勒氏激素(AMH)、雌二醇(E2)水平;蛋白印迹法(WB)检测缺氧诱导因子1α(HIF-1α)、血管内皮生长因子A抗体(VEGF)蛋白水平.结果 治疗DOR的中药共有49味,以当归使用频次最高为138次;功效以补血活血的频次最高;药物组合关联规则分析甘草-当归、当归-甘草、山药-当归常见;基础方核心药物由山药、山茱萸、炒白术、熟地黄、枸杞子、川芎、当归、白芍、甘草、香附组成.动物实验证实,与空白组比较,模型组大鼠动情周期紊乱,各级卵泡数量显著降低,血清E2显著升高、AMH显著降低,卵巢中HIF-1α、VEGF表达显著升高(P＜0.01);与模型组比较,中药基础方高剂量组以上指标均发生逆转(P＜0.01).结论 挖掘吴献群教授治疗DOR基础药物确之有效,其机制可能是通过HIF-1α/VEGF信号通路提高卵泡生成数.
Abstract
Objective Exploring the medication pattern of Professor Wu Xianqun in treating ovarian reserve dysfunction(DOR)and ver-ifying it through animal experiments.Methods Building a database based on the ancient and modern medical case cloud platform,col-lecting DOR cases treated by Wu Xianqun from 2019 to 2021,analyzing medication patterns,and screening basic prescriptions.On this basis,DOR model rats were given basic formula intervention,and the ovulation cycle of the model rats was observed through vaginal smears;Evaluation of ovarian function using eosin hematoxylin(HE)staining;Enzyme linked immunosorbent assay(ELISA)was used to detect serum levels of anti Maitreya hormone(AMH)and estradiol(E2);Western blot(WB)was used to detect the protein levels of hypoxia inducible factor 1 alpha(HIF-1 alpha)and vascular endothelial growth factor A antibody(VEGF).Results There are a total of 49 traditional Chinese medicines used to treat DOR,with Angelica sinensis having the highest frequency of use at 138 times;The high-est frequency of effectiveness is in nourishing blood and promoting blood circulation;Analysis of association rules for drug combinations:licorice Angelica sinensis,Angelica sinensis licorice,and yam Angelica sinensis are common;The core drugs of the basic formula consist of yam,Cornus officinalis,stir fried Atractylodes macrocephala,Rehmannia glutinosa,Goji berries,Chuanxiong,Angelica sinensis,Paeonia lactiflora,licorice,and Aconitum carmichaelii.Animal experiments have confirmed that compared with the blank group,the model group rats have disrupted estrous cycles,significantly reduced the number of follicles at all levels,significantly increased serum E2,significant-ly decreased AMH,and significantly increased the expression of HIF-1α and VEGF in the ovaries(P＜0.01);Compared with the model group,the high-dose group of traditional Chinese medicine basic formula showed a reversal of the above indicators(P＜0.01).Conclusion The core drug used by Professor Wu Xianqun to treat DOR is indeed effective,and its mechanism may be to increase follicle production through the HIF-1α/VEGF signaling pathway.
关键词
卵巢储备功能下降/数据挖掘/中医药治疗/古今医案云平台/实验验证Key words
Decreased ovarian reserve/Data mining/Chinese medicine treatment/Ancient and modern medical record cloud platform引用本文复制引用
出版年
2024
NETL
NSTL
万方数据