Study on the Mechanism of PTEN/PI3K/Akt/mTOR Pathway of Jiawei Qihuang Yin in Improving Diabetic Nephropathy
Objective To study the mechanism of Jiawei Qihuang Yin in improving diabetic nephropathy(DN)through PTEN/phosphatidylinositol 3 kinase(PI3K)/protein kinase B(Akt)/mechanistic target of rapamycin(mTOR)pathway.Methods Sprague Dawle(SD)rats were fed with high-fat diet combined with intraperitoneal injection of streptozotocin(STZ)to establish a DN model.The rats were divided by completely random method into model group,low-dose group,middle-dose group,high-dose group of Jiawei Qihuang Yin and losartan group.There were 10 rats in each group.According to the conversion of clinical dosage,the low-dose group,middle-dose group and high-dose group of Jiawei Qihuang Yin were set(crude drug content:200,400,800 mg·kg-1·d-1.And the blank group and model group were given 0.9%sodium chloride injection by gavage.After 8 weeks,the levels of 24-hour urine protein,serum creatinine(SCr),and blood urea nitrogen(BUN)were measured.The pathological changes of kidney were observed by Hematoxylin-eosin(HE)staining,and the protein expressions of PTEN,PI3K,Akt and mTOR in renal tissue were detected by immunohistochemistry.Results Compared with the blank group,the levels of 24 h urine protein,SCr and BUN in the model group were significantly increased(P<0.0001),and the renal function indexes in the model group were lower than those in the model group after intervention with Jiawei Qihuang Yin(P<0.001).Immunohistochemical results showed that the expression of PTEN in the model group decreased,while the expression of PI3K,Akt,and mTOR increased(P<0.01).After the intervention of Jiawei Qihuang Yin,the expression of PTEN was increased,while the expression of PI3K,Akt and mTOR decreased,with statistical significances(P<0.05).There was no significant difference between the high-dose group of Jiawei Qihuang Drink and the losartan group(P>0.05).Conclusion Jiawei Qihuang Yin may delay the development of DN through the PTEN/PI3K/Akt/mTOR pathway.
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