Exploring the Mechanism of Bian Tong Ling Capsules in Treating Post-stroke Constipation Based on Network Pharmacology
Objective To explore the mechanism of Bian Tong Ling Capsules in treating post-stroke constipation(PSC)based on network pharmacology.Methods Potential active components and targets of Bian Tong Ling Capsules were screened using the traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)and the bioinformatics analysis tool for molecular mechanisms of traditional Chinese medicine(BATMAN-TCM).PSC targets were obtained from the GeneCards datebase and online Mendelian inheritance in man(OMIM),and the intersecting targets between the drug and PSC were identified.Cytoscape 3.7.2 software was used to construct a network diagram of active component-target-disease interactions.The STRING database was utilized to build a protein-protein interaction(PPI)network of the targets,and Metascape datebase was applied for Kyoto encyclopedia of genes and genomes(KEGG)and gene ontology(GO)enrichment analyses.Results A total of 189 components of the herbal medicine and 882 disease targets were collected,with 31 intersecting targets between the drug and PSC identified.The PPI network indicated that the core targets mainly involved apoptosis,chemotaxis,inflammation,cell cycle,and proliferation.GO enrichment analysis mainly included protease binding,integrin binding,response to lipopolysaccharide,negative regulation of cell proliferation,membrane rafts,and extracellular matrix.KEGG enrichment analysis primarily involved lipid and atherosclerosis signaling pathways,relaxin signaling pathway,and cancer pathways.Conclusion The mechanism of Bian Tong Ling Capsules in treating PSC is characterized by multiple components,multiple targets,and multiple pathways.
NETL
NSTL
万方数据
