Objective To clarify the relationships between the SLCO1A2 candidate single-nucleotide polymorphisms(SNPs)rs7967354 and rs11045995 genotypes and age of breast cancer onset,as well as potential associations between the SNPs and susceptibility to breast cancer.Methods A total of 97 patients with breast cancer who visited Henan Provincial People's Hospital from March 2021 to January 2024 were included as the observation group,and 107 healthy women who visited Henan Provincial People's Hospital during the same period were included as the control group to conduct a single-center case-control study.The venous blood samples of the two groups were collected and the whole genome deoxyribonucleic acid(DNA)was extracted.The samples were amplified by polymerase chain reaction(PCR)and pyrosequencing was performed to obtain the genotypes.The differences of genotype and allele distribution frequency between the two groups were compared,and the characteristics of age distribution of two candidate SNP polymorphisms of SLCO1A2 gene and their association with breast cancer susceptibility were analyzed.Results No significant differences were found in the genotypes or allele distribution frequencies of the two candidate SLCO1A2 SNPs(P>0.05).In the breast cancer patients,the rs7967354 polymorphism was observed to be associated with the age of breast cancer onset.Furthermore,the age distributions of the wild-type and heterozygous mutant carriers of rs7967354 showed significant differences(χ2=4.556,P=0.033).Conclusion There may be no association between the two candidate SNP polymorphisms of SLCO1A2 gene and breast cancer susceptibility,and rs7967354 mutant genotype carriers may induce early onset of breast cancer.
维普
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