Preliminary Study on the Expression and Role of IDO1 in Hepatocellular Carcinoma Tissue
Objective To investigate the expression and role of indoleamine 2,3-dioxygenase 1(IDO1)in liver cancer and adjacent tissues.Methods The expression data of IDO1 in pancarcinoma and hepatocellular carcinoma were obtained by applying public databases.Immunofluorescence staining was used to detect IDO1 expression level in 82 cases of hepatocellular carcinoma,paired adjacent tissues and 14 cases of unpaired hepatocellular carcinoma.Subcutaneous transplantation tumor model of nude mice was established by subcutaneous inoculation of human hepatocellular carcinoma cells SK-HEP1,and IDO1 inhibitor was applied to intervene.Effects of IDO1 inhibitor on the growth rate of hepatocellular carcinoma cells in vivo was evaluated by hematoxylin-eosin(HE)pathological staining and proliferating cell nuclear antigen(PCNA)immunohistochemical staining.The infiltration of cluster of differentiation(CD)4+T cells and CD8+T cells in tumor tissues was detected by immunofluorescence staining.Results IDO1 was highly expressed in various carcinoma tissues.The expression level of IDO1 in hepatocellular carcinoma tissues was significantly higher than that in paracancer tissues.Pathological staining results showed that inhibition of IDO1 expression could significantly reduce the expression level of PCNA and increase the infiltration of CD4+T cells and CD8+T cells in liver cancer transplanted tumor tissues.Conclusion IDO1 is highly expressed in liver cancer tissues,and down-regulation of IDO1 expression can inhibit the proliferation of liver cancer cells in vivo,delay the growth rate of subcutaneous tumors,and enhance the response of immune cells to tumor cells.
万方数据
维普
