首页|ZEB1-3'UTR促进乳腺癌细胞MCF-7迁移机制的研究

ZEB1-3'UTR促进乳腺癌细胞MCF-7迁移机制的研究

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对与ZEB1-3'UTR结合的microR-NAs 进行生物信息学分析.结果表明,与对照组相比,过表达ZEB1-3'UTR后 MCF-7 细胞迁移能力显著增强.采用 RNA22、Microrna、Targetscan数据库筛选出与ZEB1-3'UTR结合的microRNAs,三个数据库公共预测的microRNAs有 9 个,分别是miR-429、miR-200b、miR-200c、miR-494、miR-873、miR-381、miR-300、miR-431 和 miR-342,其中 miR-429、miR-200b、miR-200c、miR-342 在乳腺癌中高表达.以上研究表明,ZEB1-3'UTR 能够竞争性结合肿瘤细胞表达的内源性microRNAs,促进了 MCF-7 细胞迁移.
Study on the Mechanism of Zeb1-3'UTR Promoting the Migration of Breast Cancer Cells Mcf-7
The combination of ZEB1-3'UTR and microRNAs was analyzed by bioinformatics.Human breast cancer cell line MCF-7 was transfected with ZEB1-3'UTR expression vector,and the migration abil-ity of breast cancer cells was ana-lyzed by Wound healing assay and transwell assay.The database RNA22,Microrna and Targetscan were used to predict the binding microRNAs of ZEB1-3'UTR,and the expression of these microRNAs in breast cancer was analyzed by UALCAN website.The results showed that compared with the control group,the migration ability of MCF-7cells was significantly enhanced after overexpression of ZEB1-3'UTR.The mi-croRNAs that binding to ZEB1-3'UTR were screened out by RNA22,Microrna and Targetscan database.Nine microRNAs highly expressed in breast cancer were pre-dicted by three databases,namely miR-429,miR-200b,miR-200c,miR-494,miR-873,miR-381,miR-300,miR-431and miR-342.Among them,miR-429,miR-200b,miR-200c and miR-342.The research showed that ZEB1-3'UTR competitively binded to endogenous microRNAs expressed by tumor cells,and further promoted the migration of MCF-7cells.

microRNAZEB1-3'UTRbioinformatics

邓英姿、孙煜曦、李一菲、张美玲、张运峰、许可、胡芬

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华北理工大学 生命科学学院,河北 唐山 063210

唐山师范学院 生命科学系,河北 唐山 063000

microRNA ZEB1-3'UTR 生物信息学

河北省省级科技计划河北省省级科技计划河北省省级科技计划唐山市科技计划唐山师范学院科研项目

H2021209007B202210501521374301D21130201C2022B09

2024

唐山师范学院学报
唐山师范学院

唐山师范学院学报

影响因子:0.204
ISSN:1009-9115
年,卷(期):2024.46(3)
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