摘要
对与ZEB1-3'UTR结合的microR-NAs 进行生物信息学分析.结果表明,与对照组相比,过表达ZEB1-3'UTR后 MCF-7 细胞迁移能力显著增强.采用 RNA22、Microrna、Targetscan数据库筛选出与ZEB1-3'UTR结合的microRNAs,三个数据库公共预测的microRNAs有 9 个,分别是miR-429、miR-200b、miR-200c、miR-494、miR-873、miR-381、miR-300、miR-431 和 miR-342,其中 miR-429、miR-200b、miR-200c、miR-342 在乳腺癌中高表达.以上研究表明,ZEB1-3'UTR 能够竞争性结合肿瘤细胞表达的内源性microRNAs,促进了 MCF-7 细胞迁移.
Abstract
The combination of ZEB1-3'UTR and microRNAs was analyzed by bioinformatics.Human breast cancer cell line MCF-7 was transfected with ZEB1-3'UTR expression vector,and the migration abil-ity of breast cancer cells was ana-lyzed by Wound healing assay and transwell assay.The database RNA22,Microrna and Targetscan were used to predict the binding microRNAs of ZEB1-3'UTR,and the expression of these microRNAs in breast cancer was analyzed by UALCAN website.The results showed that compared with the control group,the migration ability of MCF-7cells was significantly enhanced after overexpression of ZEB1-3'UTR.The mi-croRNAs that binding to ZEB1-3'UTR were screened out by RNA22,Microrna and Targetscan database.Nine microRNAs highly expressed in breast cancer were pre-dicted by three databases,namely miR-429,miR-200b,miR-200c,miR-494,miR-873,miR-381,miR-300,miR-431and miR-342.Among them,miR-429,miR-200b,miR-200c and miR-342.The research showed that ZEB1-3'UTR competitively binded to endogenous microRNAs expressed by tumor cells,and further promoted the migration of MCF-7cells.
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