Study on the Mechanism of Zeb1-3'UTR Promoting the Migration of Breast Cancer Cells Mcf-7
The combination of ZEB1-3'UTR and microRNAs was analyzed by bioinformatics.Human breast cancer cell line MCF-7 was transfected with ZEB1-3'UTR expression vector,and the migration abil-ity of breast cancer cells was ana-lyzed by Wound healing assay and transwell assay.The database RNA22,Microrna and Targetscan were used to predict the binding microRNAs of ZEB1-3'UTR,and the expression of these microRNAs in breast cancer was analyzed by UALCAN website.The results showed that compared with the control group,the migration ability of MCF-7cells was significantly enhanced after overexpression of ZEB1-3'UTR.The mi-croRNAs that binding to ZEB1-3'UTR were screened out by RNA22,Microrna and Targetscan database.Nine microRNAs highly expressed in breast cancer were pre-dicted by three databases,namely miR-429,miR-200b,miR-200c,miR-494,miR-873,miR-381,miR-300,miR-431and miR-342.Among them,miR-429,miR-200b,miR-200c and miR-342.The research showed that ZEB1-3'UTR competitively binded to endogenous microRNAs expressed by tumor cells,and further promoted the migration of MCF-7cells.