首页|6-BA对观赏水草黑木蕨组织培养的影响

6-BA对观赏水草黑木蕨组织培养的影响

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在建立黑木蕨的组培体系的基础上,探讨了外植体选择和无菌条件在组培过程中的重要性,分析了 6-BA浓度对黑木蕨出芽率、繁殖系数、死亡率和生长状态的影响.结果表明,以黑木蕨的叶柄或茎段为外植体,在MS+0.1 mg·L-1 NAA培养基中添加1.0 mg·L-1 6-BA,黑木蕨处于最佳生长状态.
Influence of 6-BA on the Tissue Culture of Ornamental Aquatic Plant Blechnum niponicum
On the basis of establishing the tissue culture system of Blechnum niponicum,this article explores the importance of selecting explants and sterile conditions in the tissue culture process,and analy-zes the effects of 6-BA concentration on the germination rate,reproductive coefficient,mortality rate,and growth status of B.niponicum.The results showed that using the petioles or stem segments of B.niponicum as explants,adding 1.0 mg·L-1 6-BA to MS+0.1 mg·L-1 NAA medium resulted in the op-timal growth state of B.niponicum.

Blechnum niponicumornamental aquatic planttissue culture6-BA

王蕊、张文洋、吴士静、刘海英、周家琪

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唐山市农业科学研究院蔬菜研究所,河北唐山 063000

唐山师范学院生命科学系,河北唐山 063000

黑木蕨 观赏水草 组织培养 6-BA

2024

唐山师范学院学报
唐山师范学院

唐山师范学院学报

影响因子:0.204
ISSN:1009-9115
年,卷(期):2024.46(6)