唐山师范学院学报2024,Vol.46Issue(6) :47-50,73.DOI:10.3969/j.issn.1009-9115.2024.06.011

枯草芽孢杆菌XE-2的分离鉴定及生物学特性分析

Isolation,Identification and Biological Characteristics Analysis of Bacillus subtilis in Soil

杜丽娜 檀冰凝 任桐瑶 徐一帆 李亚子 许可 张运峰 范永山
唐山师范学院学报2024,Vol.46Issue(6) :47-50,73.DOI:10.3969/j.issn.1009-9115.2024.06.011

枯草芽孢杆菌XE-2的分离鉴定及生物学特性分析

Isolation,Identification and Biological Characteristics Analysis of Bacillus subtilis in Soil

杜丽娜 1檀冰凝 1任桐瑶 1徐一帆 1李亚子 1许可 1张运峰 1范永山1
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作者信息

  • 1. 唐山师范学院生命科学系,河北省植物生物技术研究与应用重点实验室,河北唐山 063000
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摘要

从唐山市丰润区某中学校园内田地根际土壤中分离出枯草芽孢杆菌XE-2,再对其进行生长曲线测定、药物敏感性试验以及抑菌试验.结果表明,枯草芽孢杆菌XE-2培养0-1h为生长延迟期,1-16 h为生长对数期,16-24 h为生长平稳期.XE-2对头孢吡肟、利福平和苯唑西林等14种药物均敏感,对头孢他啶和克林霉素中介,对蜡样芽孢杆菌、大肠杆菌和苏云金杆菌均有一定的抑制作用.

Abstract

This experiment conducted growth curve determination,drug sensitivity test,and antibac-terial test on Bacillus subtilis XE-2 isolated from the field rhizosphere soil of a middle school campus in Fengrun District,Tangshan City.The results of the growth curve indicated that B.subtilis XE-2 has a de-lay period of 0-1 h,a logarithmic period of 1-16 h,and a stationary period of 16-24 h.The drug sensitivity test results showed that XE-2 is sensitive to 14 drugs,including cefepime,rifampicin,and oxacillin,and mediates with ceftazidime and clindamycin.The antibacterial test results showed that XE-2 has inhibitory effects on Bacillus cereus,Escherichia coli,and Bacillus thuringiensis.

关键词

枯草芽孢杆菌/16S/rDNA/药敏试验/抑菌试验/生长曲线

Key words

Bacillus subtilis/16S rDNA/drug sensitivity test/antibacterial test/growth curve

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出版年

2024
唐山师范学院学报
唐山师范学院

唐山师范学院学报

影响因子:0.204
ISSN:1009-9115
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