This experiment conducted growth curve determination,drug sensitivity test,and antibac-terial test on Bacillus subtilis XE-2 isolated from the field rhizosphere soil of a middle school campus in Fengrun District,Tangshan City.The results of the growth curve indicated that B.subtilis XE-2 has a de-lay period of 0-1 h,a logarithmic period of 1-16 h,and a stationary period of 16-24 h.The drug sensitivity test results showed that XE-2 is sensitive to 14 drugs,including cefepime,rifampicin,and oxacillin,and mediates with ceftazidime and clindamycin.The antibacterial test results showed that XE-2 has inhibitory effects on Bacillus cereus,Escherichia coli,and Bacillus thuringiensis.