摘要
通过 Pubchem、ETCM、SuperPred、SwissTargetPrediction 和 GEO 数据库获取积雪草酸、木犀草素、宫颈癌的交集靶点,构建蛋白互作网络图,并进行GO功能和KEGG通路富集分析.采用Cy-toscape软件构建"药物—靶点—通路"网络机制图并进行分子对接.结果发现积雪草酸、木犀草素、宫颈癌共133个交集靶点、299项GO功能和60条KEGG信号通路,6个核心靶点(IL1B、CXCL8、CASP8、CCL2、MMP9、PRKCB),分子对接的结合能均＜-5.0 kcal·mol-1,说明积雪草酸联合木犀草素能够通过多靶点、多通路发挥抗宫颈癌的作用.
Abstract
Intersection targets for asiatic acid,luteolin,and cervical cancer were obtained from Pub-Chem,ETCM,SuperPred,SwissTargetPrediction,and GEO databases,protein-protein interaction net-work was constructed,and GO functional and KEGG pathway enrichment analyses were performed.The"Drug-Target-Pathway"network mechanism diagram was built using Cytoscape software,and conducted molecular docking.Results revealed 133 intersecting targets for asiatic acid,luteolin,and cervical cancer,involving 299 GO functions and 60 KEGG signaling pathways,with six core targets identified(IL1B,CX-CL8,CASP8,CCL2,MMP9,and PRKCB).Molecular docking binding energies were less than-5.0 kcal/mol.The results indicate that asiatic acid combined with luteolin can exert anti-cervical cancer effects through multiple targets and pathways.
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