Expression and Identification of a IL-2R α Binding Sites Modified roviIL-2 in Pichia Pastoris
IL-2 is mainly produced by activated T lymphocyte, act as a natural immune enhancer, and have been widely using in the clinical setting. In previous study we constructed a recombinant ovine IL-2 expression vector pPIC9K-roIL2 apply to Pichia pastoris eukaryotic expression system. PPIC9K-roIL2 was transformed into Pichia pastoris GS115, and transformation clones were selected and named as GS115- roIL2. Products were harvested after incubation and induction in flask. Latterly the products were identified by SDS-PAGE. It showed that roIL2 was successfully expressed, and this would provide basis for the further study of immune mechanism of roviIL-2.
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