Study on the Expelling Water Retention with Drastic Purgative Effect of Euphorbia Before and After Vinegar Processing Based on the Water Load Model
Absrtact:To study the effect of Euphorbia kansui expelling water retention with drastic purgative before and after vinegar processing by establishing an animal model of water load,so as to provide a theoretical basis for the clinical treatment of ascites diseases.Using the meta-bolic cage method,rats were intraperitoneally injected with 5 mL normal saline and gavage with 5 mL normal saline to create a water load model.The effects of crude Kansui Radix group and vinegar stir-baked Euphorbia kansui group on the total urine volume of water-load rats at 0～2 h,2～4 h,4～6 h and 6 h were observed.The levels of Na+and K+in serum,AQP9 in liver and AQP2 in kidney were detected.His-tomorphology of kidney and duodenum was observed.Compared with the blank group,the urine volume,kidney index,liver AQP9 and kid-ney AQP2 level of the water load model group were significantly increased.Compared with the model group,the SH group could signifi-cantly increase 4～6 h urine volume and 6 h total urine volume in water-load model rats.The CH group significantly increased the urine vol-ume at 2～4 h and 4～6 h,which showed a continuous diuretic effect after 2 h of water loading,and the stronger the diuretic effect became in the later stage.In addition,SH group significantly improved renal balloon space and vessel wall thickening,significantly increased duodenal epithelial vascular dilatation and hyperemia,CH group significantly decreased intestinal wall purulent exudation.And the morphology of the CL group was very similar to that of the positive group.Both crude Euphorbia kansui and vinegar stir-baked Euphorbia kansui groups have significant diuretic effects on water load model rats,but the latter effect is more persistent and slow.It was preliminarily concluded that kan-sui may regulate the down-regulation of aquaporin in the kidney of water-loaded rats,affect the secretion of relevant body fluids,and inhibit water reabsorption,so as to contribute to urination.Considering that the effect of removing water is similar and the side effects on intestinal wall and kidney tissue are lower,the clinical application of vinegar stir-baked Euphorbia kansui recommended.
Euphorbia kansuiexpelling water retention with drastic purgativewater load
万方数据
维普
